Simplified gluten detection approach in the presence of a black hole quencher (BHQ)

S. Nevarez Diaz, O. Yadid-Pecht, V. Vij, J. Turner

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Optical biosensors based in the fluorescence resonance energy transfer (FRET) phenomenon require of fluorophores. The use of fluorophores requires of a complex optical system that often leads to signal loss. Thus, a new approach based in a Black Hole Quencher (BHQ) that further simplifies the biosensor is sought. This approach potentially increases the sensitivity and accuracy. For the development of this approach, the gliadin conjugate with BHQ-10 must be established first. In the present work, we used the physical phenomenon of FRET to study the gliadin conjugation with BHQ-10 molecule. We performed an experiment with a fluorophore (6-Carboxyfluorescein or 6-FAM) labeled aptamer and non-covalently attached to graphene oxide (GO). The gliadin from gluten was conjugated with BHQ using two different cross-linking reagents present in BHQ: BHQ-10 succinimidyl ester and BHQ-10 carboxylic acid. BHQ-10 with carboxylic acid as the cross-linking reagent demonstrated to be an efficient reaction for gliadin conjugation with BHQ-10. The average quenching efficiency obtained was 62% in comparison to the gliadin as a control experiment. This sets the basis for the breakthrough development of a simplified gluten detection biosensor based on absorbance measurements instead of different wavelength ranges as fluorophores do. The aim is to develop a point-of-care microfluidic system that measures gliadin from gluten in a sensitive, accurate and cost-efficient manner.

Original languageEnglish
Title of host publicationOptics in Health Care and Biomedical Optics XI
EditorsQingming Luo, Xingde Li, Ying Gu, Dan Zhu
ISBN (Electronic)9781510646490
StatePublished - 1 Jan 2021
Externally publishedYes
EventOptics in Health Care and Biomedical Optics XI 2021 - Nantong, China
Duration: 10 Oct 202112 Oct 2021

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


ConferenceOptics in Health Care and Biomedical Optics XI 2021


  • Biosensor
  • Black Hole Quencher
  • CMOS
  • Fluorescence
  • Gluten
  • Intensity
  • Microfluidic system
  • Spectrophotometer

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


Dive into the research topics of 'Simplified gluten detection approach in the presence of a black hole quencher (BHQ)'. Together they form a unique fingerprint.

Cite this