TY - JOUR
T1 - Simultaneous stereoselective high-performance liquid chromatographic determination of 10-hydroxycarbazepine and its metabolite carbamazepine- 10,11-trans-dihydrodiol in human urine
AU - Volosov, Andrew
AU - Bialer, Meir
AU - Xiaodong, Sun
AU - Perucca, Emilio
AU - Sintov, Amnon
AU - Yagen, Boris
N1 - Funding Information:
This study was supported in part by the Szold Research Fund and the Horowitz Fund. The authors wish to thank Novartis for providing DHD and MHD standards. This article was abstracted from the doctoral thesis of Andrew Volosov, as a partial fulfillment of the doctoral requirements of The Hebrew University of Jerusalem.
PY - 2000/2/11
Y1 - 2000/2/11
N2 - An enantioselective HPLC method for the simultaneous determination of the concentration of the enantiomers of the oxcarbazepine metabolites 10- hydroxycarbazepine (MHD) and carbamazepine-10,11-trans-dihydrodiol (DHD) in human urine is described. The method is based on extraction with tert.- butylmethyl ether-dichloromethane (2:1, v/v) under alkaline conditions, separation and evaporation of the organic phase and dissolution of the residue in the mobile phase. Enantiomers are resolved on a Diacel Chiralcel OD column (250 mmx4.6 mm I.D.) under isocratic conditions using as mobile phase n-hexane-ethanol-2-propanol (18:2:1, v/v/v) with addition of glacial acetic acid (0.1%). The enantiomers are detected by UV at 215 nm. The method allows reliable determination of the MHD and DHD enantiomers in human urine with limits of quantification of 0.2 mg/l and 0.4 mg/l, respectively. (C) 2000 Elsevier Science B.V.
AB - An enantioselective HPLC method for the simultaneous determination of the concentration of the enantiomers of the oxcarbazepine metabolites 10- hydroxycarbazepine (MHD) and carbamazepine-10,11-trans-dihydrodiol (DHD) in human urine is described. The method is based on extraction with tert.- butylmethyl ether-dichloromethane (2:1, v/v) under alkaline conditions, separation and evaporation of the organic phase and dissolution of the residue in the mobile phase. Enantiomers are resolved on a Diacel Chiralcel OD column (250 mmx4.6 mm I.D.) under isocratic conditions using as mobile phase n-hexane-ethanol-2-propanol (18:2:1, v/v/v) with addition of glacial acetic acid (0.1%). The enantiomers are detected by UV at 215 nm. The method allows reliable determination of the MHD and DHD enantiomers in human urine with limits of quantification of 0.2 mg/l and 0.4 mg/l, respectively. (C) 2000 Elsevier Science B.V.
KW - 10-Hydroxycarbazepine
KW - Carbamazepine-10,11-trans-dihydrodiol
UR - http://www.scopus.com/inward/record.url?scp=0033974458&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(99)00531-9
DO - 10.1016/S0378-4347(99)00531-9
M3 - Article
AN - SCOPUS:0033974458
SN - 1387-2273
VL - 738
SP - 419
EP - 425
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 2
ER -