Simultaneous stereoselective high-performance liquid chromatographic determination of 10-hydroxycarbazepine and its metabolite carbamazepine- 10,11-trans-dihydrodiol in human urine

Andrew Volosov, Meir Bialer, Sun Xiaodong, Emilio Perucca, Amnon Sintov, Boris Yagen

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

An enantioselective HPLC method for the simultaneous determination of the concentration of the enantiomers of the oxcarbazepine metabolites 10- hydroxycarbazepine (MHD) and carbamazepine-10,11-trans-dihydrodiol (DHD) in human urine is described. The method is based on extraction with tert.- butylmethyl ether-dichloromethane (2:1, v/v) under alkaline conditions, separation and evaporation of the organic phase and dissolution of the residue in the mobile phase. Enantiomers are resolved on a Diacel Chiralcel OD column (250 mmx4.6 mm I.D.) under isocratic conditions using as mobile phase n-hexane-ethanol-2-propanol (18:2:1, v/v/v) with addition of glacial acetic acid (0.1%). The enantiomers are detected by UV at 215 nm. The method allows reliable determination of the MHD and DHD enantiomers in human urine with limits of quantification of 0.2 mg/l and 0.4 mg/l, respectively. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)419-425
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume738
Issue number2
DOIs
StatePublished - 11 Feb 2000

Keywords

  • 10-Hydroxycarbazepine
  • Carbamazepine-10,11-trans-dihydrodiol

ASJC Scopus subject areas

  • General Chemistry

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