Single Fluorinated Agent for Multiplexed 19F-MRI with Micromolar Detectability Based on Dynamic Exchange

Ronit Shusterman-Krush, Nishanth D. Tirukoti, Anil Kumar Bandela, Liat Avram, Hyla Allouche-Arnon, Xiaoyang Cai, Bruce C. Gibb, Amnon Bar-Shir

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The weak thermal polarization of nuclear spins limits the sensitivity of MRI, even for MR-sensitive nuclei as fluorine-19. Therefore, despite being the source of inspiration for the development of background-free MRI for various applications, including for multiplexed imaging, the inability to map very low concentrations of targets using 19F-MRI raises the need to further enhance this platform's capabilities. Here, we employ the principles of CEST-MRI in 19F-MRI to obtain a 900-fold signal amplification of a biocompatible fluorinated agent, which can be presented in a “multicolor” fashion. Capitalizing on the dynamic interactions in host–guest supramolecular assemblies in an approach termed GEST, we demonstrate that an inhalable fluorinated anesthetic can be used as a single 19F-probe for the concurrent detection of micromolar levels of two targets, with potential in vivo translatability. Further extending GEST with new designs could expand the applicability of 19F-MRI to the mapping of targets that have so-far remained non-detectable.

Original languageEnglish
Pages (from-to)15405-15411
Number of pages7
JournalAngewandte Chemie - International Edition
Volume60
Issue number28
DOIs
StatePublished - 5 Jul 2021
Externally publishedYes

Keywords

  • CEST
  • F-MRI
  • GEST
  • host–guest systems
  • multicolor MRI

ASJC Scopus subject areas

  • Catalysis
  • Chemistry (all)

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