Single molecule detection at surfaces: Dual-color fluorescence fluctuation spectroscopy with total internal reflection excitation

M. Leutenegger, K. Hassler, P. Rigler, A. Bilenca, T. Lasser

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

Fluorescence fluctuation spectroscopy based on an evanescent field excitation scheme leads to an alternative concept for single molecule detection with interesting experimental features. This concept is described and analyzed regarding the total fluorescence process including excitation, fluorescence emission and collection close to a dielectric interface. The realized experimental scheme showed superior performance of this concept with substantially enhanced molecular brightness when compared to the classical confocal setup based on water immersion objectives. Selected applications in life sciences including dual-color surface fluorescence correlation spectroscopy, enzyme kinetics and receptor-ligand binding are underlining the interest of this experimental approach.

Original languageEnglish
Title of host publicationNew Approaches in Biomedical Spectroscopy
PublisherAmerican Chemical Society
Pages259-279
Number of pages21
ISBN (Print)0841274371, 9780841274372
DOIs
StatePublished - 1 Jan 2007
Externally publishedYes

Publication series

NameACS Symposium Series
Volume963
ISSN (Print)0097-6156

ASJC Scopus subject areas

  • Chemistry (all)
  • Chemical Engineering (all)

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