Abstract
Two experiments are described that examine the properties of a single DNA molecule. The experimental system is based on optical tweezers and on a protocol that allows to attach one end of the DNA molecule to the bottom of the sample and the other to a bead of micron size. First, the reaction of the RecA protein with DNA is monitored in a particular DNA molecule. This is made possible by the fact that the DNA is locally extended by a factor of 1.5 whenever a RecA molecule binds. Second, we monitor the dynamics of a DNA molecule as it relaxes from a fully stretched state to the coil configuration.
Original language | English |
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Pages (from-to) | 616-620 |
Number of pages | 5 |
Journal | Physica E: Low-Dimensional Systems and Nanostructures |
Volume | 9 |
Issue number | 3 |
DOIs | |
State | Published - 1 Jan 2001 |
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Condensed Matter Physics