Single-molecule studies of DNA and DNA-protein interactions

Mario Feingold

doi:10.1016/S1386-9477(00)00270-8

Single-molecule studies of DNA and DNA-protein interactions

Mario Feingold

Department of Physics

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Two experiments are described that examine the properties of a single DNA molecule. The experimental system is based on optical tweezers and on a protocol that allows to attach one end of the DNA molecule to the bottom of the sample and the other to a bead of micron size. First, the reaction of the RecA protein with DNA is monitored in a particular DNA molecule. This is made possible by the fact that the DNA is locally extended by a factor of 1.5 whenever a RecA molecule binds. Second, we monitor the dynamics of a DNA molecule as it relaxes from a fully stretched state to the coil configuration.

Original language	English
Pages (from-to)	616-620
Number of pages	5
Journal	Physica E: Low-Dimensional Systems and Nanostructures
Volume	9
Issue number	3
DOIs	https://doi.org/10.1016/S1386-9477(00)00270-8
State	Published - 1 Jan 2001

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Condensed Matter Physics

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10.1016/S1386-9477(00)00270-8

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title = "Single-molecule studies of DNA and DNA-protein interactions",

abstract = "Two experiments are described that examine the properties of a single DNA molecule. The experimental system is based on optical tweezers and on a protocol that allows to attach one end of the DNA molecule to the bottom of the sample and the other to a bead of micron size. First, the reaction of the RecA protein with DNA is monitored in a particular DNA molecule. This is made possible by the fact that the DNA is locally extended by a factor of 1.5 whenever a RecA molecule binds. Second, we monitor the dynamics of a DNA molecule as it relaxes from a fully stretched state to the coil configuration.",

author = "Mario Feingold",

note = "Funding Information: We thank A. Cahana, Y. Cohen, M. Elbaum, O. Krichevsky, E. Moses, A. Libchaber, Z. Priel and G.V. Shivashankar for useful discussions. This research was supported in part by the FIRST foundation of the Israel Academy of Science and Humanities.",

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AB - Two experiments are described that examine the properties of a single DNA molecule. The experimental system is based on optical tweezers and on a protocol that allows to attach one end of the DNA molecule to the bottom of the sample and the other to a bead of micron size. First, the reaction of the RecA protein with DNA is monitored in a particular DNA molecule. This is made possible by the fact that the DNA is locally extended by a factor of 1.5 whenever a RecA molecule binds. Second, we monitor the dynamics of a DNA molecule as it relaxes from a fully stretched state to the coil configuration.

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Single-molecule studies of DNA and DNA-protein interactions

Abstract

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