Single molecule study of the reaction between DNA and formamide

A. J. Bhattacharyya; M. Feingold

doi:10.1016/S0039-9140(01)00493-3

Single molecule study of the reaction between DNA and formamide

A. J. Bhattacharyya, M. Feingold

Department of Physics

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The kinetics of the reaction between double stranded DNA (dsDNA) and formamide is monitored at the single DNA molecule level. We find that stretching of the DNA leads to an accelerated reaction rate and to a shift in the final equilibrium concentrations. The larger the stretching force, the faster the reaction and the larger the denatured fraction of the product DNA. The single molecule kinetics is obtained from the change in the contour length of the DNA which, in turn, is measured using optical tweezers on a microbead-single DNA molecule-cover slip construct.

Original language	English
Pages (from-to)	943-949
Number of pages	7
Journal	Talanta
Volume	55
Issue number	5
DOIs	https://doi.org/10.1016/S0039-9140(01)00493-3
State	Published - 13 Dec 2001

Keywords

DNA stretching
Formamide
Optical tweezers
Single molecule

ASJC Scopus subject areas

Analytical Chemistry

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10.1016/S0039-9140(01)00493-3

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title = "Single molecule study of the reaction between DNA and formamide",

abstract = "The kinetics of the reaction between double stranded DNA (dsDNA) and formamide is monitored at the single DNA molecule level. We find that stretching of the DNA leads to an accelerated reaction rate and to a shift in the final equilibrium concentrations. The larger the stretching force, the faster the reaction and the larger the denatured fraction of the product DNA. The single molecule kinetics is obtained from the change in the contour length of the DNA which, in turn, is measured using optical tweezers on a microbead-single DNA molecule-cover slip construct.",

keywords = "DNA stretching, Formamide, Optical tweezers, Single molecule",

author = "Bhattacharyya, \{A. J.\} and M. Feingold",

note = "Funding Information: We thank A. Berman, S. Efrima, O. Krichevsky, A. Libchaber and Z. Priel for useful discussions and S. Efrima, S. Kolusheva and R. Jelinek for help with the UV measurements. This research was supported in part by the FIRST foundation of the Israel Academy of Science and Humanities, The Israel Science Foundation (grant nr. 263/00) and the Ilse Katz Center for Meso and Nanoscale Science and Technology at the Ben Gurion University.",

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doi = "10.1016/S0039-9140(01)00493-3",

language = "English",

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T1 - Single molecule study of the reaction between DNA and formamide

AU - Bhattacharyya, A. J.

AU - Feingold, M.

N1 - Funding Information: We thank A. Berman, S. Efrima, O. Krichevsky, A. Libchaber and Z. Priel for useful discussions and S. Efrima, S. Kolusheva and R. Jelinek for help with the UV measurements. This research was supported in part by the FIRST foundation of the Israel Academy of Science and Humanities, The Israel Science Foundation (grant nr. 263/00) and the Ilse Katz Center for Meso and Nanoscale Science and Technology at the Ben Gurion University.

PY - 2001/12/13

Y1 - 2001/12/13

N2 - The kinetics of the reaction between double stranded DNA (dsDNA) and formamide is monitored at the single DNA molecule level. We find that stretching of the DNA leads to an accelerated reaction rate and to a shift in the final equilibrium concentrations. The larger the stretching force, the faster the reaction and the larger the denatured fraction of the product DNA. The single molecule kinetics is obtained from the change in the contour length of the DNA which, in turn, is measured using optical tweezers on a microbead-single DNA molecule-cover slip construct.

AB - The kinetics of the reaction between double stranded DNA (dsDNA) and formamide is monitored at the single DNA molecule level. We find that stretching of the DNA leads to an accelerated reaction rate and to a shift in the final equilibrium concentrations. The larger the stretching force, the faster the reaction and the larger the denatured fraction of the product DNA. The single molecule kinetics is obtained from the change in the contour length of the DNA which, in turn, is measured using optical tweezers on a microbead-single DNA molecule-cover slip construct.

KW - DNA stretching

KW - Formamide

KW - Optical tweezers

KW - Single molecule

UR - https://www.scopus.com/pages/publications/0035856732

U2 - 10.1016/S0039-9140(01)00493-3

DO - 10.1016/S0039-9140(01)00493-3

M3 - Article

AN - SCOPUS:0035856732

SN - 0039-9140

VL - 55

SP - 943

EP - 949

JO - Talanta

JF - Talanta

IS - 5

ER -

Single molecule study of the reaction between DNA and formamide

Abstract

Keywords

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