Site-specific vibrational dynamics of the CD3ζ membrane peptide using heterodyned two-dimensional infrared photon echo spectroscopy

Prabuddha Mukherjee, Amber T. Krummel, Eric C. Fulmer, Itamar Kass, Isaiah T. Arkin, Martin T. Zanni

Research output: Contribution to journalArticlepeer-review

112 Scopus citations

Abstract

The amide I vibrational dynamics of a 27 residue peptide in lipid vesicles that encompasses the transmembrane domain of the T-cell receptor CD3 ζ was studied using a heterodyned two-dimensional (2D IR) infrered spectroscopy. The amide I mode of the 49-Leucine residue was spectroscopically isolated using 1- 13C= 18O isotope labeling. The homogeneous and inhomogeneous linewidths of this mode was measured by fitting the 2D IR spectrum collected with a photon echo pulse sequence. The population relaxation time of the amide I band was also measured with a transient grating, which was found to contribute 9 cm -1 to the linewidth.

Original languageEnglish
Pages (from-to)10215-10224
Number of pages10
JournalJournal of Chemical Physics
Volume120
Issue number21
DOIs
StatePublished - 1 Jun 2004
Externally publishedYes

ASJC Scopus subject areas

  • General Physics and Astronomy
  • Physical and Theoretical Chemistry

Fingerprint

Dive into the research topics of 'Site-specific vibrational dynamics of the CD3ζ membrane peptide using heterodyned two-dimensional infrared photon echo spectroscopy'. Together they form a unique fingerprint.

Cite this