Abstract
The amide I vibrational dynamics of a 27 residue peptide in lipid vesicles that encompasses the transmembrane domain of the T-cell receptor CD3 ζ was studied using a heterodyned two-dimensional (2D IR) infrered spectroscopy. The amide I mode of the 49-Leucine residue was spectroscopically isolated using 1- 13C= 18O isotope labeling. The homogeneous and inhomogeneous linewidths of this mode was measured by fitting the 2D IR spectrum collected with a photon echo pulse sequence. The population relaxation time of the amide I band was also measured with a transient grating, which was found to contribute 9 cm -1 to the linewidth.
Original language | English |
---|---|
Pages (from-to) | 10215-10224 |
Number of pages | 10 |
Journal | Journal of Chemical Physics |
Volume | 120 |
Issue number | 21 |
DOIs | |
State | Published - 1 Jun 2004 |
Externally published | Yes |
ASJC Scopus subject areas
- General Physics and Astronomy
- Physical and Theoretical Chemistry