Size and size distribution of subfertile human spermatozoa

N. Lauffer; S. Segal; M. Ron; N. B. Grover

doi:10.1016/s0015-0282(16)43458-8

Size and size distribution of subfertile human spermatozoa

N. Lauffer, S. Segal, M. Ron, N. B. Grover

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

An improved apparatus for measuring the electrical size (shape factor times volume) of particles based on the Coulter counter principle was used to characterize 110 subfertile semen specimens; size distributions were attainable in the 97 cases with a count above 1 x10⁶ spermatozoa/ml. The typical size distribution was unimodal and positively skewed. Spermatozoa maintained a constant profile during their passage through the orifice of the sizing apparatus, with an effective shape factor of 1.28. Two groups of cells were found. The larger one consisted of 68 specimens with a modal volume of 18.1 ± 2.0 cu μm and a cell count of 27.5 ± 9.2 x 10⁶ spermatozoa/ml (mean ± 1 SD). The smaller group contained 29 cases and had a modal volume of 6.6 ± 0.9 cu μm and a count of 10.2 ± 7.8 x10⁶. Samples with cell counts below 10 x 10⁶ spermatozoa/ml tended to have low modal volumes (less than one-half the normal), but those above showed no particular trend.

Original language	English
Pages (from-to)	188-191
Number of pages	4
Journal	Fertility and Sterility
Volume	30
Issue number	2
DOIs	https://doi.org/10.1016/s0015-0282(16)43458-8
State	Published - 1 Jan 1978
Externally published	Yes

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

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10.1016/s0015-0282(16)43458-8

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title = "Size and size distribution of subfertile human spermatozoa",

abstract = "An improved apparatus for measuring the electrical size (shape factor times volume) of particles based on the Coulter counter principle was used to characterize 110 subfertile semen specimens; size distributions were attainable in the 97 cases with a count above 1 x106 spermatozoa/ml. The typical size distribution was unimodal and positively skewed. Spermatozoa maintained a constant profile during their passage through the orifice of the sizing apparatus, with an effective shape factor of 1.28. Two groups of cells were found. The larger one consisted of 68 specimens with a modal volume of 18.1 ± 2.0 cu μm and a cell count of 27.5 ± 9.2 x 106 spermatozoa/ml (mean ± 1 SD). The smaller group contained 29 cases and had a modal volume of 6.6 ± 0.9 cu μm and a count of 10.2 ± 7.8 x106. Samples with cell counts below 10 x 106 spermatozoa/ml tended to have low modal volumes (less than one-half the normal), but those above showed no particular trend.",

author = "N. Lauffer and S. Segal and M. Ron and Grover, {N. B.}",

note = "Funding Information: Received March 13, 1978; accepted April 13, 1978. *Supported in part by a grant from the Joint Research Fund of the Hebrew University and Hadassah. tReprint requests: Neri Laufer, M.D., Department of Obstetrics and Gynecology, Hadassah University Hospital, Jerusalem, Israel. +Department of Obstetrics and Gynecology, Hadassah University Hospital. §Department of Experimental Medicine and Cancer Research, Hebrew University-Hadassah Medical School.",

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doi = "10.1016/s0015-0282(16)43458-8",

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AU - Segal, S.

AU - Ron, M.

AU - Grover, N. B.

N1 - Funding Information: Received March 13, 1978; accepted April 13, 1978. *Supported in part by a grant from the Joint Research Fund of the Hebrew University and Hadassah. tReprint requests: Neri Laufer, M.D., Department of Obstetrics and Gynecology, Hadassah University Hospital, Jerusalem, Israel. +Department of Obstetrics and Gynecology, Hadassah University Hospital. §Department of Experimental Medicine and Cancer Research, Hebrew University-Hadassah Medical School.

PY - 1978/1/1

Y1 - 1978/1/1

N2 - An improved apparatus for measuring the electrical size (shape factor times volume) of particles based on the Coulter counter principle was used to characterize 110 subfertile semen specimens; size distributions were attainable in the 97 cases with a count above 1 x106 spermatozoa/ml. The typical size distribution was unimodal and positively skewed. Spermatozoa maintained a constant profile during their passage through the orifice of the sizing apparatus, with an effective shape factor of 1.28. Two groups of cells were found. The larger one consisted of 68 specimens with a modal volume of 18.1 ± 2.0 cu μm and a cell count of 27.5 ± 9.2 x 106 spermatozoa/ml (mean ± 1 SD). The smaller group contained 29 cases and had a modal volume of 6.6 ± 0.9 cu μm and a count of 10.2 ± 7.8 x106. Samples with cell counts below 10 x 106 spermatozoa/ml tended to have low modal volumes (less than one-half the normal), but those above showed no particular trend.

AB - An improved apparatus for measuring the electrical size (shape factor times volume) of particles based on the Coulter counter principle was used to characterize 110 subfertile semen specimens; size distributions were attainable in the 97 cases with a count above 1 x106 spermatozoa/ml. The typical size distribution was unimodal and positively skewed. Spermatozoa maintained a constant profile during their passage through the orifice of the sizing apparatus, with an effective shape factor of 1.28. Two groups of cells were found. The larger one consisted of 68 specimens with a modal volume of 18.1 ± 2.0 cu μm and a cell count of 27.5 ± 9.2 x 106 spermatozoa/ml (mean ± 1 SD). The smaller group contained 29 cases and had a modal volume of 6.6 ± 0.9 cu μm and a count of 10.2 ± 7.8 x106. Samples with cell counts below 10 x 106 spermatozoa/ml tended to have low modal volumes (less than one-half the normal), but those above showed no particular trend.

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Size and size distribution of subfertile human spermatozoa

Abstract

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