Slow depolarizing afterpotentials in neocortical neurons are sodium and calcium dependent

A. Friedman; J. Arens; U. Heinemann; M. J. Gutnick

doi:10.1016/0304-3940(92)90125-Q

Slow depolarizing afterpotentials in neocortical neurons are sodium and calcium dependent

A. Friedman, J. Arens, U. Heinemann, M. J. Gutnick

Department of Physiology and Cell Biology

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Depolarizing afterpotentials (DAPs) were studied in intracellular recordings from neocortical slices bathed in tetrodotoxin (TTX) (1 μM) and tetraethylammonium chloride (TEA) (24 mM), to block voltage-dependent Na⁺ currents and most K⁺ currents. The DAP was Ca²⁺-dependent, in that its magnitude varied as a function of the duration of the preceding Ca²⁺ plateau. It had an apparent reversal potential of between -40 and -5 mV. The DAP was blocked when choline replaced all extracellular Na⁺; there was a hyperpolarizing shift in apparent reversal potential when extracellular Na⁺ was lowered. The DAP was blocked by amiloride (1 mM), which also decreased the preceding Ca²⁺ plateau. The data are consistent with the hypothesis that the DAP is due to electrogenic Na⁺ Ca²⁺ exchange.

Original language	English
Pages (from-to)	13-17
Number of pages	5
Journal	Neuroscience Letters
Volume	135
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(92)90125-Q
State	Published - 20 Jan 1992

Keywords

Brain slice
Calcium spike
Depolarizing afterpotential
Guinea pig
Intracellular recording
Na Ca exchanger
Neocortex

ASJC Scopus subject areas

General Neuroscience

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0304-3940(92)90125-Q

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title = "Slow depolarizing afterpotentials in neocortical neurons are sodium and calcium dependent",

abstract = "Depolarizing afterpotentials (DAPs) were studied in intracellular recordings from neocortical slices bathed in tetrodotoxin (TTX) (1 μM) and tetraethylammonium chloride (TEA) (24 mM), to block voltage-dependent Na+ currents and most K+ currents. The DAP was Ca2+-dependent, in that its magnitude varied as a function of the duration of the preceding Ca2+ plateau. It had an apparent reversal potential of between -40 and -5 mV. The DAP was blocked when choline replaced all extracellular Na+; there was a hyperpolarizing shift in apparent reversal potential when extracellular Na+ was lowered. The DAP was blocked by amiloride (1 mM), which also decreased the preceding Ca2+ plateau. The data are consistent with the hypothesis that the DAP is due to electrogenic Na+ Ca2+ exchange.",

keywords = "Brain slice, Calcium spike, Depolarizing afterpotential, Guinea pig, Intracellular recording, Na Ca exchanger, Neocortex",

author = "A. Friedman and J. Arens and U. Heinemann and Gutnick, {M. J.}",

note = "Funding Information: This work was supported by a grant from the Israel National Council for Research and Development and the Commission for the European Communities, and a grant from the DGF (SFB 194).",

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doi = "10.1016/0304-3940(92)90125-Q",

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AU - Friedman, A.

AU - Arens, J.

AU - Heinemann, U.

AU - Gutnick, M. J.

N1 - Funding Information: This work was supported by a grant from the Israel National Council for Research and Development and the Commission for the European Communities, and a grant from the DGF (SFB 194).

PY - 1992/1/20

Y1 - 1992/1/20

N2 - Depolarizing afterpotentials (DAPs) were studied in intracellular recordings from neocortical slices bathed in tetrodotoxin (TTX) (1 μM) and tetraethylammonium chloride (TEA) (24 mM), to block voltage-dependent Na+ currents and most K+ currents. The DAP was Ca2+-dependent, in that its magnitude varied as a function of the duration of the preceding Ca2+ plateau. It had an apparent reversal potential of between -40 and -5 mV. The DAP was blocked when choline replaced all extracellular Na+; there was a hyperpolarizing shift in apparent reversal potential when extracellular Na+ was lowered. The DAP was blocked by amiloride (1 mM), which also decreased the preceding Ca2+ plateau. The data are consistent with the hypothesis that the DAP is due to electrogenic Na+ Ca2+ exchange.

AB - Depolarizing afterpotentials (DAPs) were studied in intracellular recordings from neocortical slices bathed in tetrodotoxin (TTX) (1 μM) and tetraethylammonium chloride (TEA) (24 mM), to block voltage-dependent Na+ currents and most K+ currents. The DAP was Ca2+-dependent, in that its magnitude varied as a function of the duration of the preceding Ca2+ plateau. It had an apparent reversal potential of between -40 and -5 mV. The DAP was blocked when choline replaced all extracellular Na+; there was a hyperpolarizing shift in apparent reversal potential when extracellular Na+ was lowered. The DAP was blocked by amiloride (1 mM), which also decreased the preceding Ca2+ plateau. The data are consistent with the hypothesis that the DAP is due to electrogenic Na+ Ca2+ exchange.

KW - Brain slice

KW - Calcium spike

KW - Depolarizing afterpotential

KW - Guinea pig

KW - Intracellular recording

KW - Na Ca exchanger

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JO - Neuroscience Letters

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Slow depolarizing afterpotentials in neocortical neurons are sodium and calcium dependent

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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