Solid-State 2H NMR Study of Thymidine. Base Rigidity and Ribose Ring Flexibility in Deoxynucleosides

Yukio Hiyama; Siddhartha Roy; Jack S. Cohen; Dennis A. Torchia

doi:10.1021/ja00205a008

Solid-State ²H NMR Study of Thymidine. Base Rigidity and Ribose Ring Flexibility in Deoxynucleosides

Yukio Hiyama, Siddhartha Roy, Jack S. Cohen, Dennis A. Torchia

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

We have investigated the internal motions of the nucleoside thymidine in the solid state by deuterium NMR spectroscopy. The base position was found to be essentially rigid, even at elevated temperatures. On the other hand, T₁ measurements on 2', 2”-dideuteriothymidine indicated the presence of small-amplitude motions on the nanosecond time scale. In addition, spin alignment and distorted echo experiments revealed the presence of large-amplitude motion on millisecond to microsecond time scales. This motion is hypothesized to be 2'-endo ↔ 3'-endo interconversion. It was also shown that the large-amplitude motion may be very sensitive to the crystal packing forces.

Original language	English
Pages (from-to)	8609-8613
Number of pages	5
Journal	Journal of the American Chemical Society
Volume	111
Issue number	23
DOIs	https://doi.org/10.1021/ja00205a008
State	Published - 1 Jan 1989
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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title = "Solid-State 2H NMR Study of Thymidine. Base Rigidity and Ribose Ring Flexibility in Deoxynucleosides",

abstract = "We have investigated the internal motions of the nucleoside thymidine in the solid state by deuterium NMR spectroscopy. The base position was found to be essentially rigid, even at elevated temperatures. On the other hand, T1 measurements on 2', 2”-dideuteriothymidine indicated the presence of small-amplitude motions on the nanosecond time scale. In addition, spin alignment and distorted echo experiments revealed the presence of large-amplitude motion on millisecond to microsecond time scales. This motion is hypothesized to be 2'-endo ↔ 3'-endo interconversion. It was also shown that the large-amplitude motion may be very sensitive to the crystal packing forces.",

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AU - Hiyama, Yukio

AU - Roy, Siddhartha

AU - Cohen, Jack S.

AU - Torchia, Dennis A.

PY - 1989/1/1

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N2 - We have investigated the internal motions of the nucleoside thymidine in the solid state by deuterium NMR spectroscopy. The base position was found to be essentially rigid, even at elevated temperatures. On the other hand, T1 measurements on 2', 2”-dideuteriothymidine indicated the presence of small-amplitude motions on the nanosecond time scale. In addition, spin alignment and distorted echo experiments revealed the presence of large-amplitude motion on millisecond to microsecond time scales. This motion is hypothesized to be 2'-endo ↔ 3'-endo interconversion. It was also shown that the large-amplitude motion may be very sensitive to the crystal packing forces.

AB - We have investigated the internal motions of the nucleoside thymidine in the solid state by deuterium NMR spectroscopy. The base position was found to be essentially rigid, even at elevated temperatures. On the other hand, T1 measurements on 2', 2”-dideuteriothymidine indicated the presence of small-amplitude motions on the nanosecond time scale. In addition, spin alignment and distorted echo experiments revealed the presence of large-amplitude motion on millisecond to microsecond time scales. This motion is hypothesized to be 2'-endo ↔ 3'-endo interconversion. It was also shown that the large-amplitude motion may be very sensitive to the crystal packing forces.

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JF - Journal of the American Chemical Society

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ER -

Solid-State ²H NMR Study of Thymidine. Base Rigidity and Ribose Ring Flexibility in Deoxynucleosides

Abstract

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