Solid-supported synthesis of 5′-mRNA CAP-4 from trypanosomatids

Magdalena Lewdorowicz, Jacek Jemielity, Ryszard Kierzek, Michal Shapira, Janusz Stepinski, Edward Darzynkiewicz

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


The unique structure of 5′ mRNA cap from Trypanosomatids is the most modified cap found in nature. Here we present the synthesis of cap-4 (m 7 Gpppm36,6,2′ Apm2′ Apm2′ Cpm23,2′ Up) on a disulfide-tethered solid support. This approach allows obtaining cap-4 more efficiently then previously described. Moreover such modified resin could be a useful tool for affinity purification of Leishmania proteins interacting with cap-4. For the final step of synthesis, namely coupling of phosphorylated tetranucleotide with activated 7-methylguanosine 5′-diphosphate two systems were compared. Surprisingly, the coupling in water with Mn2+ as a catalyst, gave better results than usually more effective coupling in DMF with ZnCl2.

Original languageEnglish
Pages (from-to)1329-1333
Number of pages5
JournalNucleosides, Nucleotides and Nucleic Acids
Issue number10-12
StatePublished - 1 Oct 2007


  • 5′ mRNA Cap-4
  • Disulfide linker
  • Leishmania
  • Oligonucleotide synthesis
  • Solid support

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics


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