SP1 protein-based nanostructures and arrays

Izhar Medalsy; Or Dgany; Mukhles Sowwan; Hezy Cohen; Alevtyna Yukashevska; Sharon G. Wolf; Amnon Wolf; Abraham Koster; Orna Almog; Ira Marton; Yehonathan Pouny; Arie Altman; Oded Shoseyov; Danny Porath

doi:10.1021/nl072455t

SP1 protein-based nanostructures and arrays

Izhar Medalsy, Or Dgany, Mukhles Sowwan, Hezy Cohen, Alevtyna Yukashevska, Sharon G. Wolf, Amnon Wolf, Abraham Koster, Orna Almog, Ira Marton, Yehonathan Pouny, Arie Altman, Oded Shoseyov, Danny Porath

Research output: Contribution to journal › Article › peer-review

70 Scopus citations

Abstract

Controlled formation of complex nanostructures is one of the main goals of nanoscience and nanotechnology. Stable Protein 1 (SP1) is a boiling-stable ring protein complex, 11 nm in diameter, which self-assembles from 12 identical monomers. SP1 can be utilized to form large ordered arrays; it can be easily modified by genetic engineering to produce various mutants; it is also capable of binding gold nanoparticles (GNPs) and thus forming protein-GNP chains made of alternating SP1s and GNPs. We report the formation and the protocols leading to the formation of those nanostructures and their characterization by transmission electron microscopy, atomic force microscopy, and electrostatic force microscopy. Further control over the GNP interdistances within the protein-GNP chains may lead to the formation of nanowires and structures that may be useful for nanoelectronics.

Original language	English
Pages (from-to)	473-477
Number of pages	5
Journal	Nano Letters
Volume	8
Issue number	2
DOIs	https://doi.org/10.1021/nl072455t
State	Published - 1 Feb 2008

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

Access to Document

10.1021/nl072455t

Cite this

@article{1599b2e8402b4ad689babffc0b1c782f,

title = "SP1 protein-based nanostructures and arrays",

abstract = "Controlled formation of complex nanostructures is one of the main goals of nanoscience and nanotechnology. Stable Protein 1 (SP1) is a boiling-stable ring protein complex, 11 nm in diameter, which self-assembles from 12 identical monomers. SP1 can be utilized to form large ordered arrays; it can be easily modified by genetic engineering to produce various mutants; it is also capable of binding gold nanoparticles (GNPs) and thus forming protein-GNP chains made of alternating SP1s and GNPs. We report the formation and the protocols leading to the formation of those nanostructures and their characterization by transmission electron microscopy, atomic force microscopy, and electrostatic force microscopy. Further control over the GNP interdistances within the protein-GNP chains may lead to the formation of nanowires and structures that may be useful for nanoelectronics.",

author = "Izhar Medalsy and Or Dgany and Mukhles Sowwan and Hezy Cohen and Alevtyna Yukashevska and Wolf, {Sharon G.} and Amnon Wolf and Abraham Koster and Orna Almog and Ira Marton and Yehonathan Pouny and Arie Altman and Oded Shoseyov and Danny Porath",

year = "2008",

month = feb,

day = "1",

doi = "10.1021/nl072455t",

language = "English",

volume = "8",

pages = "473--477",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society",

number = "2",

}

TY - JOUR

T1 - SP1 protein-based nanostructures and arrays

AU - Medalsy, Izhar

AU - Dgany, Or

AU - Sowwan, Mukhles

AU - Cohen, Hezy

AU - Yukashevska, Alevtyna

AU - Wolf, Sharon G.

AU - Wolf, Amnon

AU - Koster, Abraham

AU - Almog, Orna

AU - Marton, Ira

AU - Pouny, Yehonathan

AU - Altman, Arie

AU - Shoseyov, Oded

AU - Porath, Danny

PY - 2008/2/1

Y1 - 2008/2/1

N2 - Controlled formation of complex nanostructures is one of the main goals of nanoscience and nanotechnology. Stable Protein 1 (SP1) is a boiling-stable ring protein complex, 11 nm in diameter, which self-assembles from 12 identical monomers. SP1 can be utilized to form large ordered arrays; it can be easily modified by genetic engineering to produce various mutants; it is also capable of binding gold nanoparticles (GNPs) and thus forming protein-GNP chains made of alternating SP1s and GNPs. We report the formation and the protocols leading to the formation of those nanostructures and their characterization by transmission electron microscopy, atomic force microscopy, and electrostatic force microscopy. Further control over the GNP interdistances within the protein-GNP chains may lead to the formation of nanowires and structures that may be useful for nanoelectronics.

AB - Controlled formation of complex nanostructures is one of the main goals of nanoscience and nanotechnology. Stable Protein 1 (SP1) is a boiling-stable ring protein complex, 11 nm in diameter, which self-assembles from 12 identical monomers. SP1 can be utilized to form large ordered arrays; it can be easily modified by genetic engineering to produce various mutants; it is also capable of binding gold nanoparticles (GNPs) and thus forming protein-GNP chains made of alternating SP1s and GNPs. We report the formation and the protocols leading to the formation of those nanostructures and their characterization by transmission electron microscopy, atomic force microscopy, and electrostatic force microscopy. Further control over the GNP interdistances within the protein-GNP chains may lead to the formation of nanowires and structures that may be useful for nanoelectronics.

UR - http://www.scopus.com/inward/record.url?scp=40449133607&partnerID=8YFLogxK

U2 - 10.1021/nl072455t

DO - 10.1021/nl072455t

M3 - Article

AN - SCOPUS:40449133607

SN - 1530-6984

VL - 8

SP - 473

EP - 477

JO - Nano Letters

JF - Nano Letters

IS - 2

ER -

SP1 protein-based nanostructures and arrays

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this