Spatial mismatch between the Na+ flux and spike initiation in axon initial segment

Gytis Baranauskas, Yaron David, Ilya A. Fleidervish

Research output: Contribution to journalArticlepeer-review

76 Scopus citations


It is widely believed that, in cortical pyramidal cells, action potentials (APs) initiate in the distal portion of axon initial segment (AIS) because that is where Na+ channel density is highest. To investigate the relationship between the density of Na+ channels and the spatiotemporal pattern of AP initiation, we simultaneously recorded Na+ flux and action currents along the proximal axonal length. We found that functional Na+ channel density is approximately four times lower in the AP trigger zone than in the middle of the AIS, where it is highest. Computational analysis of AP initiation revealed a paradoxical mismatch between the AP threshold and Na+ channel density, which could be explained by the lopsided capacitive load imposed on the proximal end of the AIS by the somatodendritic compartment. Favorable conditions for AP initiation are therefore achieved in the distal AIS portion, close to the edge of myelin, where the current source-load ratio is highest. Our findings suggest that cable properties play a central role in determining where the AP starts, such that small plastic changes in the local AIS Na+ channel density could have a large influence on neuronal excitability as a whole.

Original languageEnglish
Pages (from-to)4051-4056
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number10
StatePublished - 5 Mar 2013


  • Neocortex
  • Pyramidal neuron
  • Sodium imaging

ASJC Scopus subject areas

  • General


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