TY - JOUR
T1 - Spatiotemporal analysis of F-actin polymerization with micropillar arrays reveals synchronization between adhesion sites
AU - Hollander, Sarit
AU - Guo, Yuanning
AU - Wolfenson, Haguy
AU - Zaritsky, Assaf
N1 - Publisher Copyright:
© 2024 Hollander et al. This article is distributed by The American Society for CellBiology under license from the author(s). It is available to the public under an Attribution 4.0 International Creative Commons CC-BY 4.0 License (https://creativecommons.org/licenses/by/4.0/).
PY - 2024/12/1
Y1 - 2024/12/1
N2 - We repurposed micropillar arrays to quantify spatiotemporal inter-adhesion communication. Following the observation that integrin adhesions formed around pillar tops we relied on the precise repetitive spatial control of the pillars to reliably monitor F-actin dynamics in mouse embryonic fibroblasts as a model for spatiotemporal adhesion–related intracellular signaling. Using correlation-based analyses, we revealed localized information flows propagating between adjacent pillars that were integrated over space and time to synchronize the adhesion dynamics within the entire cell. Probing the mechanical regulation, we discovered that stiffer pillars or partial actomyosin contractility inhibition enhances inter-adhesion F-actin synchronization, and that inhibition of Arp2/3, but not formin, reduces synchronization. Our results suggest that adhesions can communicate and highlight the potential of using micropillar arrays as a tool to measure spatiotemporal intracellular signaling.
AB - We repurposed micropillar arrays to quantify spatiotemporal inter-adhesion communication. Following the observation that integrin adhesions formed around pillar tops we relied on the precise repetitive spatial control of the pillars to reliably monitor F-actin dynamics in mouse embryonic fibroblasts as a model for spatiotemporal adhesion–related intracellular signaling. Using correlation-based analyses, we revealed localized information flows propagating between adjacent pillars that were integrated over space and time to synchronize the adhesion dynamics within the entire cell. Probing the mechanical regulation, we discovered that stiffer pillars or partial actomyosin contractility inhibition enhances inter-adhesion F-actin synchronization, and that inhibition of Arp2/3, but not formin, reduces synchronization. Our results suggest that adhesions can communicate and highlight the potential of using micropillar arrays as a tool to measure spatiotemporal intracellular signaling.
UR - https://www.scopus.com/pages/publications/85208602063
U2 - 10.1091/mbc.E24-06-0276
DO - 10.1091/mbc.E24-06-0276
M3 - Article
C2 - 39441710
AN - SCOPUS:85208602063
SN - 1059-1524
VL - 35
JO - Molecular Biology of the Cell
JF - Molecular Biology of the Cell
IS - 12
M1 - br23
ER -