TY - JOUR
T1 - Specific activity of carp antisera against β2-microglobulin (β2m) and evidence for a β2m homologue in carp (Cyprinus carpio)
AU - Shalev, A.
AU - Caspi, R. R.
AU - Lögdberg, L.
AU - Björck, L.
AU - Segal, S.
AU - Avtalion, R. R.
N1 - Funding Information:
This study was supported by the Fund for Basic Research administrated by the Israel Academy of Sciences and Humanities (Dr. A. Shalev), by KGSS Geesthacht-Tesperhude, Germany (Dr. R. Avtalion), and by the Swedish Medical Research Council (Project No. 6456; Dr. L. Bj6rck and Dr. L. L6gdberg). The authors thank Dr. M.D. Poulik (William Beaumont Hospital, Royal Oak, Michigan) and Dr. A. Fucks (McGill Univ., Montreal) for providing the rabbit anti-dog ~2m antiserum and the horse anti-human B2m antiserum.
PY - 1984/1/1
Y1 - 1984/1/1
N2 - Antisera against rabbit and human β2-microglobulin were produced in carp (Cyprinus carpio). These antisera were found to be specific for β2m, and detected β2m epitopes in various vertebrates including guinea-pig, cow, mouse, rat, dog, horse, cat, sheep, goat, parrot, chicken and frog. In addition, these antisera were also inhibited by an extract from oyster. The differences between the reactivity of these two antisera and the ability of the anti-rabbit β2m to distinguish between mouse and rat β2m's suggested that the carp itself carries β2m epitopes. This hypothesis was corroborated by the ability of a variety of mammalian anti-β2m antisera to induce a mitogenic response in carp leukocytes. Finally, a rabbit antiserum against dog β2m was shown to precipitate a low molecular weight molecule from carp leukocyte extract. This molecule is likely to represent the carp homologue of mammalian β2m.
AB - Antisera against rabbit and human β2-microglobulin were produced in carp (Cyprinus carpio). These antisera were found to be specific for β2m, and detected β2m epitopes in various vertebrates including guinea-pig, cow, mouse, rat, dog, horse, cat, sheep, goat, parrot, chicken and frog. In addition, these antisera were also inhibited by an extract from oyster. The differences between the reactivity of these two antisera and the ability of the anti-rabbit β2m to distinguish between mouse and rat β2m's suggested that the carp itself carries β2m epitopes. This hypothesis was corroborated by the ability of a variety of mammalian anti-β2m antisera to induce a mitogenic response in carp leukocytes. Finally, a rabbit antiserum against dog β2m was shown to precipitate a low molecular weight molecule from carp leukocyte extract. This molecule is likely to represent the carp homologue of mammalian β2m.
UR - http://www.scopus.com/inward/record.url?scp=0021223097&partnerID=8YFLogxK
U2 - 10.1016/0145-305X(84)90096-X
DO - 10.1016/0145-305X(84)90096-X
M3 - Article
C2 - 6209173
AN - SCOPUS:0021223097
SN - 0145-305X
VL - 8
SP - 639
EP - 648
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - 3
ER -