Antisera against rabbit and human β2-microglobulin were produced in carp (Cyprinus carpio). These antisera were found to be specific for β2m, and detected β2m epitopes in various vertebrates including guinea-pig, cow, mouse, rat, dog, horse, cat, sheep, goat, parrot, chicken and frog. In addition, these antisera were also inhibited by an extract from oyster. The differences between the reactivity of these two antisera and the ability of the anti-rabbit β2m to distinguish between mouse and rat β2m's suggested that the carp itself carries β2m epitopes. This hypothesis was corroborated by the ability of a variety of mammalian anti-β2m antisera to induce a mitogenic response in carp leukocytes. Finally, a rabbit antiserum against dog β2m was shown to precipitate a low molecular weight molecule from carp leukocyte extract. This molecule is likely to represent the carp homologue of mammalian β2m.
ASJC Scopus subject areas
- Developmental Biology