Specificity of nucleotide binding sites in isolated chloroplast coupling factor (CF1)

Menahem Banai, Noun Shavit, David M. Chipman

Research output: Contribution to journalArticlepeer-review


The binding of various nucleotides to chloroplast coupling factor CF1 was studied by two dialysis techniques. It was found that the number of nucleoside diphosphate sites and their specificities for the base moiety is dependent on the magnesium concentration. In the presence of 50 μM added MgCl2, the protein has a single strong site/mol protein with Kd = 0.5 μM for ADP and high specificity (Kd > 20 μM for ε{lunate}ADP, GDP, CDP). In the presence of 5 mM MgCl2, the protein has two independent tight ADP sites (Kd = 0.4 μM) of low specificity (Kd ≈ 0.8, 2, and 2 μrmM, respectively for ε{lunate}ADP, GDP, and CDP). These results are compared with the specificity of the partial reactions for photophosphorylation.

Original languageEnglish
Pages (from-to)100-107
Number of pages8
JournalBiochimica et Biophysica Acta - Bioenergetics
Issue number1
StatePublished - 11 Oct 1978

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology


Dive into the research topics of 'Specificity of nucleotide binding sites in isolated chloroplast coupling factor (CF1)'. Together they form a unique fingerprint.

Cite this