TY - JOUR
T1 - Specificity of nucleotide binding sites in isolated chloroplast coupling factor (CF1)
AU - Banai, Menahem
AU - Shavit, Noun
AU - Chipman, David M.
N1 - Funding Information:
This work was supported by grant 21-A of the Israel National Academy of Sciences. We thank Mrs. R. Yaniv for technical assistance.
PY - 1978/10/11
Y1 - 1978/10/11
N2 - The binding of various nucleotides to chloroplast coupling factor CF1 was studied by two dialysis techniques. It was found that the number of nucleoside diphosphate sites and their specificities for the base moiety is dependent on the magnesium concentration. In the presence of 50 μM added MgCl2, the protein has a single strong site/mol protein with Kd = 0.5 μM for ADP and high specificity (Kd > 20 μM for ε{lunate}ADP, GDP, CDP). In the presence of 5 mM MgCl2, the protein has two independent tight ADP sites (Kd = 0.4 μM) of low specificity (Kd ≈ 0.8, 2, and 2 μrmM, respectively for ε{lunate}ADP, GDP, and CDP). These results are compared with the specificity of the partial reactions for photophosphorylation.
AB - The binding of various nucleotides to chloroplast coupling factor CF1 was studied by two dialysis techniques. It was found that the number of nucleoside diphosphate sites and their specificities for the base moiety is dependent on the magnesium concentration. In the presence of 50 μM added MgCl2, the protein has a single strong site/mol protein with Kd = 0.5 μM for ADP and high specificity (Kd > 20 μM for ε{lunate}ADP, GDP, CDP). In the presence of 5 mM MgCl2, the protein has two independent tight ADP sites (Kd = 0.4 μM) of low specificity (Kd ≈ 0.8, 2, and 2 μrmM, respectively for ε{lunate}ADP, GDP, and CDP). These results are compared with the specificity of the partial reactions for photophosphorylation.
UR - http://www.scopus.com/inward/record.url?scp=0018171202&partnerID=8YFLogxK
U2 - 10.1016/0005-2728(78)90009-9
DO - 10.1016/0005-2728(78)90009-9
M3 - Article
C2 - 152121
AN - SCOPUS:0018171202
SN - 0005-2728
VL - 504
SP - 100
EP - 107
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 1
ER -