Spectrophotometric analysis of carotenoids in plant extracts based on elimination of chlorophyll absorption

Mark N. Merzlyak, Inna Khozin, Zvi Cohen

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Subtraction of the absorption spectra of chlorophyll(s) from the total spectra of plant cell extracts reveals the carotenoid absorption spectra in both the ultraviolet and visible regions, from which the total carotenoid content can be easily evaluated. The applications of this method to Spirulina platensis cells treated with the herbicide norflurazon and Anabaena siamensis cells grown under high light irradiation are demonstrated. The possibility of subtraction of the absorption of both chlorophyll a and b is demonstrated in an extract of the leaves of a higher plant (grape).

Original languageEnglish
Pages (from-to)294-299
Number of pages6
JournalPhytochemical Analysis
Volume7
Issue number6
DOIs
StatePublished - 1 Jan 1996

Keywords

  • Anabaena siamensis
  • Carotenoids
  • Chlorophylls
  • Norflurazon
  • Spirulina platensis

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