TY - JOUR
T1 - Spectroscopic evidence for site-specific cellular activity in the tubular gland in human intestine
AU - Ramesh, Jagannathan
AU - Argov, Shmuel
AU - Salman, Ahmad
AU - Yuzhelevski, Marina
AU - Sinelnikov, Igor
AU - Goldstein, Jed
AU - Erukhimovitch, Vitaly
AU - Mordechai, Shaul
PY - 2002/3/5
Y1 - 2002/3/5
N2 - The intestinal crypts contain mucus-secreting goblet cells in large numbers. In the tubular gland (crypt), the cells are generated at the bottom and end their life cycle at the top. Recently, FTIR microspectroscopy (FTIR-MC) has been applied in biology and medicine. The characterization of various cellular types using FTIR-MC and its subsequent application for the diagnosis of cancer is becoming a reality. In this report, we investigate the differential cellular activity in the normal tubular gland using FTIR-MC. Our results indicate that the absorbance for the cells in the bottom of the crypt is always higher than those in the upper portion. There are spectral pattern changes and frequency shifts for cells at the bottom and top sites of the normal crypt. Also, the comparison of a normal crypt with a malignant one has been made. This is the first spectroscopic evidence in the literature showing the difference in the cellular activity at different sites in the tubular gland. The reasons for our observations and their implications are discussed.
AB - The intestinal crypts contain mucus-secreting goblet cells in large numbers. In the tubular gland (crypt), the cells are generated at the bottom and end their life cycle at the top. Recently, FTIR microspectroscopy (FTIR-MC) has been applied in biology and medicine. The characterization of various cellular types using FTIR-MC and its subsequent application for the diagnosis of cancer is becoming a reality. In this report, we investigate the differential cellular activity in the normal tubular gland using FTIR-MC. Our results indicate that the absorbance for the cells in the bottom of the crypt is always higher than those in the upper portion. There are spectral pattern changes and frequency shifts for cells at the bottom and top sites of the normal crypt. Also, the comparison of a normal crypt with a malignant one has been made. This is the first spectroscopic evidence in the literature showing the difference in the cellular activity at different sites in the tubular gland. The reasons for our observations and their implications are discussed.
KW - Cancer
KW - Colon
KW - Crypt epithelial cells
KW - FTIR microspectroscopy
UR - https://www.scopus.com/pages/publications/0036182219
U2 - 10.1007/s00249-001-0191-1
DO - 10.1007/s00249-001-0191-1
M3 - Article
C2 - 11908852
AN - SCOPUS:0036182219
SN - 0175-7571
VL - 30
SP - 612
EP - 616
JO - European Biophysics Journal
JF - European Biophysics Journal
IS - 8
ER -
