Sperm capacitation is, after all, a prerequisite for both partial and complete acrosome reaction

Bijay S. Jaiswal, Anat Cohen-Dayag, Ilan Tur-Kaspa, Michael Eisenbach

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

The acrosome reaction (AR) - an essential step in mammalian fertilization - can occur, according to the consensus, only in capacitated spermatozoa. In apparent contrast, recent reports have demonstrated that human spermatozoa incubated in vitro in an albumin-free medium and therefore believed to be non-capacitated do undergo the AR. With the aim of determining unequivocally whether or not capacitation is required for the AR and whether albumin is essential for capacitation, we compared the potential to undergo partial and complete AR (induced by phorbol myristate ester or by the Ca2+ ionophore A23187) between human spermatozoa incubated in a capacitating medium, albumin-free medium, and non-capacitating medium. The results clearly demonstrate that capacitation is, after all, a prerequisite for both partial and complete AR. Albumin, on the other hand, is essential only for acquiring the capacity to undergo complete, not partial AR.

Original languageEnglish
Pages (from-to)309-313
Number of pages5
JournalFEBS Letters
Volume427
Issue number2
DOIs
StatePublished - 8 May 1998
Externally publishedYes

Keywords

  • A23187
  • Acrosome reaction
  • Albumin
  • Capacitation
  • Human sperm
  • Phorbol ester

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint

Dive into the research topics of 'Sperm capacitation is, after all, a prerequisite for both partial and complete acrosome reaction'. Together they form a unique fingerprint.

Cite this