TY - JOUR
T1 - Spermatozoa retrieved by electroejaculation
T2 - Should we prefer fresh or cryopreserved spermatozoa for intracytoplasmic sperm injection?
AU - Aizer, Adva
AU - Dratviman-Storobinsky, Olga
AU - Noach-Hirsh, Meirav
AU - Konopnicki, Sarah
AU - Raviv, Gil
AU - Orvieto, Raoul
N1 - Publisher Copyright:
© 2020 Blackwell Verlag GmbH
PY - 2020/10/1
Y1 - 2020/10/1
N2 - We aim to evaluate our experience, comparing intracytoplasmic sperm injection (ICSI) outcomes of cycle using fresh versus thawed electroejaculated spermatozoa. All consecutive couples undergoing ICSI cycles using electroejaculated spermatozoa, during a 16-year period, were evaluated. Embryological/laboratory variables of the ICSI cycles were assessed and compared between those utilising fresh (fresh group) versus thawed (thawed group) electroejaculated spermatozoa. Fifty-seven couples were evaluated, 30 used a fresh electroejaculated spermatozoa in 55 ICSI cycles, while 27 used a thawed sperm sample in 41 ICSI cycles. There were no in-between group differences in the mean numbers of oocytes retrieved per oocyte retrieval nor the percentage of MII oocytes. The fresh group demonstrated significantly higher fertilisation (71.5% vs. 64.1%, respectively, p '.05), top-quality embryos (66.5% vs. 54.9%, respectively, p '.02), clinical pregnancy per transfer (41.3% and 21.2%, respectively, p '.05) and cumulative clinical pregnancy (58.2% vs. 26.8%, respectively, p '.001) rates, as compared to the thawed group. Independent of the source of spermatozoa used, no pregnancy was achieved following ICSI utilising immotile spermatozoa. In conclusion, ICSI cycles using ejaculated spermatozoa of patients suffering from neurologic or psychogenic anejaculation are reassuring. The use of fresh ejaculated spermatozoa retrieved on the day of the female spouse oocyte retrieval might improve outcome. Whenever a thawed electroejaculated spermatozoa yield no motile spermatozoa, emergency electroejaculation is mandatory.
AB - We aim to evaluate our experience, comparing intracytoplasmic sperm injection (ICSI) outcomes of cycle using fresh versus thawed electroejaculated spermatozoa. All consecutive couples undergoing ICSI cycles using electroejaculated spermatozoa, during a 16-year period, were evaluated. Embryological/laboratory variables of the ICSI cycles were assessed and compared between those utilising fresh (fresh group) versus thawed (thawed group) electroejaculated spermatozoa. Fifty-seven couples were evaluated, 30 used a fresh electroejaculated spermatozoa in 55 ICSI cycles, while 27 used a thawed sperm sample in 41 ICSI cycles. There were no in-between group differences in the mean numbers of oocytes retrieved per oocyte retrieval nor the percentage of MII oocytes. The fresh group demonstrated significantly higher fertilisation (71.5% vs. 64.1%, respectively, p '.05), top-quality embryos (66.5% vs. 54.9%, respectively, p '.02), clinical pregnancy per transfer (41.3% and 21.2%, respectively, p '.05) and cumulative clinical pregnancy (58.2% vs. 26.8%, respectively, p '.001) rates, as compared to the thawed group. Independent of the source of spermatozoa used, no pregnancy was achieved following ICSI utilising immotile spermatozoa. In conclusion, ICSI cycles using ejaculated spermatozoa of patients suffering from neurologic or psychogenic anejaculation are reassuring. The use of fresh ejaculated spermatozoa retrieved on the day of the female spouse oocyte retrieval might improve outcome. Whenever a thawed electroejaculated spermatozoa yield no motile spermatozoa, emergency electroejaculation is mandatory.
KW - electroejaculation
KW - fresh
KW - frozen-thawed
KW - intracytoplasmic sperm injection
KW - pregnancy rate
UR - http://www.scopus.com/inward/record.url?scp=85086038688&partnerID=8YFLogxK
U2 - 10.1111/and.13671
DO - 10.1111/and.13671
M3 - Article
C2 - 32510642
AN - SCOPUS:85086038688
SN - 0303-4569
VL - 52
JO - Andrologia
JF - Andrologia
IS - 9
M1 - e13671
ER -