Spin label study of the process of oxygen release by pea chloroplasts

A. V. Kulikov; Ye I. Yudanova; G. I. Likhtenshtein; S. I. Allakhverdiyev; V. V. Klimov

Spin label study of the process of oxygen release by pea chloroplasts

A. V. Kulikov, Ye I. Yudanova, G. I. Likhtenshtein, S. I. Allakhverdiyev, V. V. Klimov

Research output: Contribution to journal › Article › peer-review

Abstract

Nitroxyl fatty acid probes inserted into the chloroplast membranes have been used to study the process of evolution of oxygen by chloroplasts. From the kinetics of the reduction of the nitroxyls on exposure to continuous illumination at two values of u.h.f. power the authors have determined the products k_e[O₂] where k_e is the spin exchange constant of nitroxyl and oxygen; [O₂] is the oxygen concentration. It is shown that unlike liposomes, in the chloroplast membranes there is no oxygen in the dark. This is possible as a result of oxygen uptake in the different biochemical processes. On illumination of the chloroplasts the oxygen evolved is detected, the maximum value k_e[O₂]=1·2 × 10⁵ sec^-1 being observed at the centre of the membrane. At temperatures above 40 and below -20 °C oxygen is not evolved.

Original language	English
Pages (from-to)	1055-1060
Number of pages	6
Journal	Biophysics (Russian Federation)
Volume	33
Issue number	6
State	Published - 1 Dec 1988
Externally published	Yes

ASJC Scopus subject areas

Biophysics

Cite this

@article{b1b07fa749444f3b9be53a7a73151ca1,

title = "Spin label study of the process of oxygen release by pea chloroplasts",

abstract = "Nitroxyl fatty acid probes inserted into the chloroplast membranes have been used to study the process of evolution of oxygen by chloroplasts. From the kinetics of the reduction of the nitroxyls on exposure to continuous illumination at two values of u.h.f. power the authors have determined the products ke[O2] where ke is the spin exchange constant of nitroxyl and oxygen; [O2] is the oxygen concentration. It is shown that unlike liposomes, in the chloroplast membranes there is no oxygen in the dark. This is possible as a result of oxygen uptake in the different biochemical processes. On illumination of the chloroplasts the oxygen evolved is detected, the maximum value ke[O2]=1·2 × 105 sec-1 being observed at the centre of the membrane. At temperatures above 40 and below -20 °C oxygen is not evolved.",

author = "Kulikov, {A. V.} and Yudanova, {Ye I.} and Likhtenshtein, {G. I.} and Allakhverdiyev, {S. I.} and Klimov, {V. V.}",

year = "1988",

month = dec,

day = "1",

language = "English",

volume = "33",

pages = "1055--1060",

journal = "Biophysics (Russian Federation)",

issn = "0006-3509",

publisher = "Pleiades Publishing",

number = "6",

}

TY - JOUR

T1 - Spin label study of the process of oxygen release by pea chloroplasts

AU - Kulikov, A. V.

AU - Yudanova, Ye I.

AU - Likhtenshtein, G. I.

AU - Allakhverdiyev, S. I.

AU - Klimov, V. V.

PY - 1988/12/1

Y1 - 1988/12/1

N2 - Nitroxyl fatty acid probes inserted into the chloroplast membranes have been used to study the process of evolution of oxygen by chloroplasts. From the kinetics of the reduction of the nitroxyls on exposure to continuous illumination at two values of u.h.f. power the authors have determined the products ke[O2] where ke is the spin exchange constant of nitroxyl and oxygen; [O2] is the oxygen concentration. It is shown that unlike liposomes, in the chloroplast membranes there is no oxygen in the dark. This is possible as a result of oxygen uptake in the different biochemical processes. On illumination of the chloroplasts the oxygen evolved is detected, the maximum value ke[O2]=1·2 × 105 sec-1 being observed at the centre of the membrane. At temperatures above 40 and below -20 °C oxygen is not evolved.

AB - Nitroxyl fatty acid probes inserted into the chloroplast membranes have been used to study the process of evolution of oxygen by chloroplasts. From the kinetics of the reduction of the nitroxyls on exposure to continuous illumination at two values of u.h.f. power the authors have determined the products ke[O2] where ke is the spin exchange constant of nitroxyl and oxygen; [O2] is the oxygen concentration. It is shown that unlike liposomes, in the chloroplast membranes there is no oxygen in the dark. This is possible as a result of oxygen uptake in the different biochemical processes. On illumination of the chloroplasts the oxygen evolved is detected, the maximum value ke[O2]=1·2 × 105 sec-1 being observed at the centre of the membrane. At temperatures above 40 and below -20 °C oxygen is not evolved.

UR - http://www.scopus.com/inward/record.url?scp=45549115682&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:45549115682

SN - 0006-3509

VL - 33

SP - 1055

EP - 1060

JO - Biophysics (Russian Federation)

JF - Biophysics (Russian Federation)

IS - 6

ER -

Spin label study of the process of oxygen release by pea chloroplasts

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this