SPR Based Fiber Optic Sensor for the Detection of Vitellogenin: An Endocrine Disruption Biomarker in Aquatic Environments

Sachin K Srivastava; Roli Verma; Banshi D Gupta; Isam Khalaila; Ibrahim Abdulhalim

doi:10.4172/2090-4967.1000114

SPR Based Fiber Optic Sensor for the Detection of Vitellogenin: An Endocrine Disruption Biomarker in Aquatic Environments

Sachin K Srivastava, Roli Verma, Banshi D Gupta, Isam Khalaila, Ibrahim Abdulhalim

Department of Biotechnology Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

We have fabricated a fiber optic SPR biosensor for the detection of vitellogenin (Vg), an endocrine disruption biomarker in aquatic environments. The sensor was fabricated by immobilizing anti-Vg antibodies on the sensor surface. Control experiments performed on another similar protein, fetuin and on a sensing probe without the anti-Vg antibody receptor confirmed the specificity of the sensor. The limit of detection of the sensor was found to be as small as 1 ng/ml in our experimental window. The sensitivity of the sensor was 0.48 nm/(ng/ml). This sensor can be utilized for remote monitoring of the water bodies for any endocrine disruption phenomena.

Original language	English
Article number	1000114
Number of pages	5
Journal	Biosensors
Volume	4
Issue number	1
DOIs	https://doi.org/10.4172/2090-4967.1000114
State	Published - May 2015

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title = "SPR Based Fiber Optic Sensor for the Detection of Vitellogenin: An Endocrine Disruption Biomarker in Aquatic Environments",

abstract = "We have fabricated a fiber optic SPR biosensor for the detection of vitellogenin (Vg), an endocrine disruption biomarker in aquatic environments. The sensor was fabricated by immobilizing anti-Vg antibodies on the sensor surface. Control experiments performed on another similar protein, fetuin and on a sensing probe without the anti-Vg antibody receptor confirmed the specificity of the sensor. The limit of detection of the sensor was found to be as small as 1 ng/ml in our experimental window. The sensitivity of the sensor was 0.48 nm/(ng/ml). This sensor can be utilized for remote monitoring of the water bodies for any endocrine disruption phenomena.",

author = "Srivastava, {Sachin K} and Roli Verma and Gupta, {Banshi D} and Isam Khalaila and Ibrahim Abdulhalim",

year = "2015",

month = may,

doi = "10.4172/2090-4967.1000114",

language = "English",

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T1 - SPR Based Fiber Optic Sensor for the Detection of Vitellogenin

T2 - An Endocrine Disruption Biomarker in Aquatic Environments

AU - Srivastava, Sachin K

AU - Verma, Roli

AU - Gupta, Banshi D

AU - Khalaila, Isam

AU - Abdulhalim, Ibrahim

PY - 2015/5

Y1 - 2015/5

N2 - We have fabricated a fiber optic SPR biosensor for the detection of vitellogenin (Vg), an endocrine disruption biomarker in aquatic environments. The sensor was fabricated by immobilizing anti-Vg antibodies on the sensor surface. Control experiments performed on another similar protein, fetuin and on a sensing probe without the anti-Vg antibody receptor confirmed the specificity of the sensor. The limit of detection of the sensor was found to be as small as 1 ng/ml in our experimental window. The sensitivity of the sensor was 0.48 nm/(ng/ml). This sensor can be utilized for remote monitoring of the water bodies for any endocrine disruption phenomena.

AB - We have fabricated a fiber optic SPR biosensor for the detection of vitellogenin (Vg), an endocrine disruption biomarker in aquatic environments. The sensor was fabricated by immobilizing anti-Vg antibodies on the sensor surface. Control experiments performed on another similar protein, fetuin and on a sensing probe without the anti-Vg antibody receptor confirmed the specificity of the sensor. The limit of detection of the sensor was found to be as small as 1 ng/ml in our experimental window. The sensitivity of the sensor was 0.48 nm/(ng/ml). This sensor can be utilized for remote monitoring of the water bodies for any endocrine disruption phenomena.

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DO - 10.4172/2090-4967.1000114

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ER -

SPR Based Fiber Optic Sensor for the Detection of Vitellogenin: An Endocrine Disruption Biomarker in Aquatic Environments

Abstract

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