Stilbene molecular probes as potential materials for bioengineering: Real time analysis of antioxidants and nitric oxide, immunoassay in solution and biomembranes fluidity

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Abstract

A series of fluorescent methods of analysis and investigation of system based on the use of stilbenes and potentially important in biochemistry, biophysics, biotechnology, and biomedicine were proposed and developed. In these methods, two new types of stilbene molecular probes have been used: (i) fluorescent photochrome molecules and (ii) super molecules containing fluorescent and fluorescent quenching segments. These methods utilize the following photochemical and photophysical phenomena: The fluorescence and phosphorescence quenching, photochrome photoisomerization, and triplet-triplet and singlet-singlet energy transfer. The fluorescence properties of the new probes were intensively exploited as the basis of several methodologies that include a real-time analysis of nitric oxide, immunoassay in solution, investigation of molecular dynamics of biomembranes in a wide range characteristic times, and characterization of sensors for antibodies. These techniques may be adapted to fibro-optic sensoring.

Original languageEnglish
Title of host publicationMaterials Science and Chemical Engineering
Pages718-723
Number of pages6
DOIs
StatePublished - 8 Jul 2013
Event2013 International Conference on Materials Science and Chemical Engineering, MSCE 2013 - , Singapore
Duration: 20 Feb 201321 Feb 2013

Publication series

NameAdvanced Materials Research
Volume699
ISSN (Print)1022-6680

Conference

Conference2013 International Conference on Materials Science and Chemical Engineering, MSCE 2013
Country/TerritorySingapore
Period20/02/1321/02/13

Keywords

  • Aspartic acids
  • Biomembrane fluidity
  • Fluorescent-photochrome method
  • Immunoassay
  • Nitric oxide
  • Stilbenes
  • Triplet-triplet and singlet-singlet energy transfer

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