TY - JOUR
T1 - Stimulated-single fiber electromyography monitoring of anti-sense induced changes in experimental autoimmune myasthenia gravis
AU - Boneva, Neli
AU - Hamra-Amitay, Yasmine
AU - Wirguin, Itzhak
AU - Brenner, Talma
N1 - Funding Information:
This work was supported by grants from the Association Francaise Contre les Myopathies (AFM), France and Ester/Neuroscience, Israel. We thank Dr. Z. Argov for helpful discussion and Mrs. C. Sicsic for her excellent technical assistance.
PY - 2006/5/1
Y1 - 2006/5/1
N2 - The neuromuscular weakness associated with myasthenia gravis (MG) can be transiently relieved by pharmacological inhibitors of acetylcholinesterase (AChE). Here, we expand the anticholinesterase repertoire to include 2′-O-methyl-protected antisense oligonucleotides targeted to AChE mRNA (EN101). Using stimulated-single fiber electromyography, we show that EN101 treatment of rats with experimental autoimmune myasthenia gravis (EAMG), improved the mean consecutive difference (MCD) and blocking for 24 h. This treatment was more efficient than pyridostigmine and was accompanied by marked improvement in stamina and clinical profile.
AB - The neuromuscular weakness associated with myasthenia gravis (MG) can be transiently relieved by pharmacological inhibitors of acetylcholinesterase (AChE). Here, we expand the anticholinesterase repertoire to include 2′-O-methyl-protected antisense oligonucleotides targeted to AChE mRNA (EN101). Using stimulated-single fiber electromyography, we show that EN101 treatment of rats with experimental autoimmune myasthenia gravis (EAMG), improved the mean consecutive difference (MCD) and blocking for 24 h. This treatment was more efficient than pyridostigmine and was accompanied by marked improvement in stamina and clinical profile.
KW - Acetylcholinesterase inhibition
KW - Anti-sense treatment
KW - Experimental autoimmune myasthenia gravis
KW - Myasthenia gravis
KW - Stimulated-single fiber electromyography
UR - http://www.scopus.com/inward/record.url?scp=33646487206&partnerID=8YFLogxK
U2 - 10.1016/j.neures.2006.01.003
DO - 10.1016/j.neures.2006.01.003
M3 - Article
C2 - 16504322
AN - SCOPUS:33646487206
VL - 55
SP - 40
EP - 44
JO - Neuroscience Research
JF - Neuroscience Research
SN - 0168-0102
IS - 1
ER -