Stimulation of molt by RNA interference of the molt-inhibiting hormone in the crayfish Cherax quadricarinatus

Ramachandra R. Pamuru, Ohad Rosen, Rivka Manor, J. Sook Chung, Nilli Zmora, Lilah Glazer, Eliahu D. Aflalo, Simy Weil, Sherry L. Tamone, Amir Sagi

Research output: Contribution to journalArticlepeer-review

60 Scopus citations


In crustaceans, molting is known to be under the control of neuropeptide hormones synthesized and secreted from the eyestalk ganglia. While the role of molt-inhibiting hormone (MIH) in regulating molting has been described in several species using classical methods, an in vivo specific MIH targeted manipulation has not been described yet. In the present study, an MIH cDNA was isolated and sequenced from the eyestalk ganglia of the Australian freshwater red claw crayfish Cherax quadricarinatus (Cq) by 5' and 3' RACE. We analyzed the putative Cq-MIH based on sequence homology, a three dimensional structure model and transcript's tissue specificity. We further examined the involvement of Cq-MIH in the control of molt in the crayfish through RNAi by in vivo injections of Cq-MIH double-stranded RNA, which resulted in, similarly to eyestalk ablation, acceleration of molt cycles. This acceleration was reflected by a significant reduction (up to 32%) in molt interval and an increased rate in molt mineralization index (MMI), which correlated with the induction of ecdysteroid hormones compared to control. Altogether, this study provides a proof of function for the involvement of the Cq-MIH gene in molt regulation in the crayfish.

Original languageEnglish
Pages (from-to)227-236
Number of pages10
JournalGeneral and Comparative Endocrinology
Issue number2
StatePublished - 1 Sep 2012


  • Cherax quadricarinatus
  • DsRNA
  • Ecdysone
  • Molt cycle
  • Molt-inhibiting hormone
  • RNAi

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Endocrinology


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