Stimulation of the respiratory burst in peripheral blood monocytes by lipoteichoic acid. The involvement of calcium ions and phospholipase A2

D. Tarsi-Tsuk, R. Levy

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Lipoteichoic acid (LTA) from Streptococcus faecalis stimulates the respiratory burst in peripheral blood monocytes (mon), as measured by cytochrome C reduction. The effect of LTA was time and dose dependent. LTA stimulated the respiratory burst in a biphasic manner with a range of 1 to 1000 ng/ml·106 mon, with maximal activity at 50 ng/ml. At this concentration LTA increased the activity from 0.97 ± 0.2 to 4.88 ± 0.2 nmol·106 mon/20 min. The role of calcium ions in the effect of LTA in stimulating respiratory burst was studied by changing the availability of calcium ions in the medium, and by measuring the effect of LTA on 45Ca2+ uptake and on intracellular Ca2+ levels. Removal of extracellular calcium ions in the presence of the calcium chelator EGTA, abolished the LTA-stimulated respiratory burst. LTA (50 ng/ml) was found to increase 45Ca2+ uptake into monocytes within seconds (from 2200 ± 242 in the untreated cells to 4642 ± 365 cpm/min in the LTA-treated mon). At this concentration, LTA stimulated an immediate rise in the intracellular free Ca2+ concentration to 155 ± 15 nM as compared with 120 ± 14 nM in the unstimulated monocytes. LTA caused a specific release of arachidonic acid indicating the involvement of phospholipase A2 in the transduction signal stimulating the respiratory burst by LTA.

Original languageEnglish
Pages (from-to)2665-2670
Number of pages6
JournalJournal of Immunology
Volume144
Issue number7
StatePublished - 1 Jan 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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