Stochastic Sensing of Chloride Anions Using an α-Hemolysin Pore with a semiaza-Bambusuril Adapter

Ofer Reany; Mercedes Romero-Ruiz; Raman Khurana; Pravat Mondal; Ehud Keinan; Hagan Bayley

doi:10.1002/anie.202406719

Stochastic Sensing of Chloride Anions Using an α-Hemolysin Pore with a semiaza-Bambusuril Adapter

Ofer Reany, Mercedes Romero-Ruiz, Raman Khurana, Pravat Mondal, Ehud Keinan, Hagan Bayley

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Pores containing molecular adapters provide internal selective binding sites, thereby allowing the stochastic sensing of analytes. Herein, we demonstrate that semiaza-bambusuril (BU) acts as a non-covalent molecular adapter when lodged within the lumen of the wild-type α-hemolysin (WT-αHL) protein pore. Because the bambusurils are recognized as anion receptors, the anion binding site within the adapter-nanopore complex allows the detection of chloride anions, thus converting a non-selective pore into an anion sensor.

Original language	English
Article number	e202406719
Journal	Angewandte Chemie - International Edition
Volume	63
Issue number	39
DOIs	https://doi.org/10.1002/anie.202406719
State	Published - 23 Sep 2024
Externally published	Yes

Keywords

bambusurils
chloride anions
nanopore
stochastic sensing
α-hemolysin

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.202406719

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title = "Stochastic Sensing of Chloride Anions Using an α-Hemolysin Pore with a semiaza-Bambusuril Adapter",

abstract = "Pores containing molecular adapters provide internal selective binding sites, thereby allowing the stochastic sensing of analytes. Herein, we demonstrate that semiaza-bambusuril (BU) acts as a non-covalent molecular adapter when lodged within the lumen of the wild-type α-hemolysin (WT-αHL) protein pore. Because the bambusurils are recognized as anion receptors, the anion binding site within the adapter-nanopore complex allows the detection of chloride anions, thus converting a non-selective pore into an anion sensor.",

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T1 - Stochastic Sensing of Chloride Anions Using an α-Hemolysin Pore with a semiaza-Bambusuril Adapter

AU - Reany, Ofer

AU - Romero-Ruiz, Mercedes

AU - Khurana, Raman

AU - Mondal, Pravat

AU - Keinan, Ehud

AU - Bayley, Hagan

PY - 2024/9/23

Y1 - 2024/9/23

N2 - Pores containing molecular adapters provide internal selective binding sites, thereby allowing the stochastic sensing of analytes. Herein, we demonstrate that semiaza-bambusuril (BU) acts as a non-covalent molecular adapter when lodged within the lumen of the wild-type α-hemolysin (WT-αHL) protein pore. Because the bambusurils are recognized as anion receptors, the anion binding site within the adapter-nanopore complex allows the detection of chloride anions, thus converting a non-selective pore into an anion sensor.

AB - Pores containing molecular adapters provide internal selective binding sites, thereby allowing the stochastic sensing of analytes. Herein, we demonstrate that semiaza-bambusuril (BU) acts as a non-covalent molecular adapter when lodged within the lumen of the wild-type α-hemolysin (WT-αHL) protein pore. Because the bambusurils are recognized as anion receptors, the anion binding site within the adapter-nanopore complex allows the detection of chloride anions, thus converting a non-selective pore into an anion sensor.

KW - bambusurils

KW - chloride anions

KW - nanopore

KW - stochastic sensing

KW - α-hemolysin

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JO - Angewandte Chemie - International Edition

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Stochastic Sensing of Chloride Anions Using an α-Hemolysin Pore with a semiaza-Bambusuril Adapter

Abstract

Keywords

ASJC Scopus subject areas

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