Strategies for the preparation of homogenous glycoproteins

Ashraf Brik, Simon Ficht, Chi Huey Wong

Research output: Contribution to journalReview articlepeer-review

36 Scopus citations

Abstract

The majority of human proteins are glycosylated, and many of them are known to be involved in important biological processes. This class of proteins is often expressed as a heterogeneous mixture of glycoforms, rendering the isolation of individual species for various studies a difficult task. Recent advances in the development of glycoprotein synthesis have provided promising strategies, which include enzymatic, chemical and in vivo suppressor tRNA methods to obtain homogenous products. Nevertheless, there are many remaining challenges to overcome in both the chemical and biochemical approaches to efficiently obtain homogenous glycoproteins for glycobiology research and for the production of pharmaceutical products.

Original languageEnglish
Pages (from-to)638-644
Number of pages7
JournalCurrent Opinion in Chemical Biology
Volume10
Issue number6
DOIs
StatePublished - 1 Dec 2006
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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