TY - JOUR
T1 - Strategies for the preparation of homogenous glycoproteins
AU - Brik, Ashraf
AU - Ficht, Simon
AU - Wong, Chi Huey
N1 - Funding Information:
This work was supported by the NIH and Skaggs Institute for Chemical Biology. Simon Ficht is grateful for a postdoctoral fellowship provided by the Deutsche Akademische Austauschdienst (DAAD).
PY - 2006/12/1
Y1 - 2006/12/1
N2 - The majority of human proteins are glycosylated, and many of them are known to be involved in important biological processes. This class of proteins is often expressed as a heterogeneous mixture of glycoforms, rendering the isolation of individual species for various studies a difficult task. Recent advances in the development of glycoprotein synthesis have provided promising strategies, which include enzymatic, chemical and in vivo suppressor tRNA methods to obtain homogenous products. Nevertheless, there are many remaining challenges to overcome in both the chemical and biochemical approaches to efficiently obtain homogenous glycoproteins for glycobiology research and for the production of pharmaceutical products.
AB - The majority of human proteins are glycosylated, and many of them are known to be involved in important biological processes. This class of proteins is often expressed as a heterogeneous mixture of glycoforms, rendering the isolation of individual species for various studies a difficult task. Recent advances in the development of glycoprotein synthesis have provided promising strategies, which include enzymatic, chemical and in vivo suppressor tRNA methods to obtain homogenous products. Nevertheless, there are many remaining challenges to overcome in both the chemical and biochemical approaches to efficiently obtain homogenous glycoproteins for glycobiology research and for the production of pharmaceutical products.
UR - http://www.scopus.com/inward/record.url?scp=33751422228&partnerID=8YFLogxK
U2 - 10.1016/j.cbpa.2006.10.003
DO - 10.1016/j.cbpa.2006.10.003
M3 - Review article
AN - SCOPUS:33751422228
SN - 1367-5931
VL - 10
SP - 638
EP - 644
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
IS - 6
ER -