Structural analysis of multicellular organisms with cryo-electron tomography

Jan Harapin, Mandy Börmel, K. Tanuj Sapra, Damian Brunner, Andres Kaech, Ohad Medalia

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

We developed a method for visualizing tissues from multicellular organisms using cryo-electron tomography. Our protocol involves vitrifying samples with high-pressure freezing, thinning them with cryo-FIB-SEM (focused-ion-beam scanning electron microscopy) and applying fiducial gold markers under cryogenic conditions to the lamellae post-milling. We applied this protocol to acquire tomograms of vitrified Caenorhabditis elegans embryos and worms, which showed the intracellular organization of selected tissues at particular developmental stages in otherwise intact specimens.

Original languageEnglish
Pages (from-to)634-636
Number of pages3
JournalNature Methods
Volume12
Issue number7
DOIs
StatePublished - 30 Jun 2015

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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