Structural analysis of multicellular organisms with cryo-electron tomography

Jan Harapin; Mandy Börmel; K. Tanuj Sapra; Damian Brunner; Andres Kaech; Ohad Medalia

doi:10.1038/nmeth.3401

Structural analysis of multicellular organisms with cryo-electron tomography

Jan Harapin, Mandy Börmel, K. Tanuj Sapra, Damian Brunner, Andres Kaech, Ohad Medalia

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

84 Scopus citations

Abstract

We developed a method for visualizing tissues from multicellular organisms using cryo-electron tomography. Our protocol involves vitrifying samples with high-pressure freezing, thinning them with cryo-FIB-SEM (focused-ion-beam scanning electron microscopy) and applying fiducial gold markers under cryogenic conditions to the lamellae post-milling. We applied this protocol to acquire tomograms of vitrified Caenorhabditis elegans embryos and worms, which showed the intracellular organization of selected tissues at particular developmental stages in otherwise intact specimens.

Original language	English
Pages (from-to)	634-636
Number of pages	3
Journal	Nature Methods
Volume	12
Issue number	7
DOIs	https://doi.org/10.1038/nmeth.3401
State	Published - 30 Jun 2015

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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abstract = "We developed a method for visualizing tissues from multicellular organisms using cryo-electron tomography. Our protocol involves vitrifying samples with high-pressure freezing, thinning them with cryo-FIB-SEM (focused-ion-beam scanning electron microscopy) and applying fiducial gold markers under cryogenic conditions to the lamellae post-milling. We applied this protocol to acquire tomograms of vitrified Caenorhabditis elegans embryos and worms, which showed the intracellular organization of selected tissues at particular developmental stages in otherwise intact specimens.",

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AU - Börmel, Mandy

AU - Sapra, K. Tanuj

AU - Brunner, Damian

AU - Kaech, Andres

AU - Medalia, Ohad

PY - 2015/6/30

Y1 - 2015/6/30

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AB - We developed a method for visualizing tissues from multicellular organisms using cryo-electron tomography. Our protocol involves vitrifying samples with high-pressure freezing, thinning them with cryo-FIB-SEM (focused-ion-beam scanning electron microscopy) and applying fiducial gold markers under cryogenic conditions to the lamellae post-milling. We applied this protocol to acquire tomograms of vitrified Caenorhabditis elegans embryos and worms, which showed the intracellular organization of selected tissues at particular developmental stages in otherwise intact specimens.

UR - https://www.scopus.com/pages/publications/84934440295

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DO - 10.1038/nmeth.3401

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Structural analysis of multicellular organisms with cryo-electron tomography

Abstract

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