Structural and mechanical analysis of tectorial membrane Tecta mutants

Rachel Gueta, Jonathan Levitt, Anping Xia, Ori Katz, John S. Oghalai, Itay Rousso

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The tectorial membrane (TM) is an extracellular matrix of the cochlea whose prominent role in hearing has been demonstrated through mutation studies. The C1509G mutation of the Tecta gene, which encodes for the α-tectorin protein, leads to hearing loss. The heterozygote TM only attaches to the first row of outer hair cells (OHCs), and the homozygote TM does not attach to any OHCs. Here we measured the morphology and mechanical properties of wild-type, heterozygous, and homozygous Tecta TMs. Morphological analyses conducted with second-and third-harmonic imaging, scanning electron microscopy, and immunolabeling revealed marked changes in the collagen architecture and stereocilin-labeling patterns of the mutant TMs. The mechanical properties of the mutant TM were measured by force spectroscopy. Whereas the axial Young's modulus of the low-frequency (apical) region of Tecta mutant TM samples was similar to that of wild-type TMs, it significantly decreased in the basal region to a value approaching that found at the apex. Modeling simulations suggest that a reduced TM Young's modulus is likely to reduce OHC stereociliary deflection. These findings argue that the heterozygote C1509G mutation results in a lack of attachment of the TM to the OHCs, which in turn reduces both the overall number of OHCs that are involved in mechanotransduction and the degree of mechanotransduction exhibited by the OHCs that remain attached to the TM.

Original languageEnglish
Pages (from-to)2530-2538
Number of pages9
JournalBiophysical Journal
Volume100
Issue number10
DOIs
StatePublished - 18 May 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics

Fingerprint

Dive into the research topics of 'Structural and mechanical analysis of tectorial membrane Tecta mutants'. Together they form a unique fingerprint.

Cite this