@article{ad92f57910c74d4cb97b1d7e70ab4af3,
title = "Structural basis for histone H2B deubiquitination by the SAGA DUB module",
abstract = "Monoubiquitinated histone H2B plays multiple roles in transcription activation. H2B is deubiquitinated by the Spt-Ada-Gcn5 acetyltransferase (SAGA) coactivator, which contains a four-protein subcomplex known as the deubiquitinating (DUB) module. The crystal structure of the Ubp8/Sgf11/Sus1/Sgf73 DUB module bound to a ubiquitinated nucleosome reveals that the DUB module primarily contacts H2A/H2B, with an arginine cluster on the Sgf11 zinc finger domain docking on the conserved H2A/H2B acidic patch. The Ubp8 catalytic domain mediates additional contacts with H2B, as well as with the conjugated ubiquitin. We find that the DUB module deubiquitinates H2B both in the context of the nucleosome and in H2A/H2B dimers complexed with the histone chaperone, FACT, suggesting that SAGA could target H2B at multiple stages of nucleosome disassembly and reassembly during transcription.",
author = "Morgan, {Michael T.} and Mahmood Haj-Yahya and Ringel, {Alison E.} and Prasanthi Bandi and Ashraf Brik and Cynthia Wolberger",
note = "Funding Information: We thank T. Formosa for the generous gift of purified FACT and S. Tan for the plasmid, pST55-16x601. We thank X. Zhang for assistance with protein purification, P. Lombardi for assistance with data collection, and D. Leahy for helpful discussions. Supported by grant GM-095822 from the National Institute of General Medical Sciences (C.W.) and by a Ruth L. Kirschstein National Research Service Award (M.M.). A.B. is a Neubauer Professor and a Taub Foundation Fellow. GM/CA @ APS has been funded by the National Cancer Institute (Y1-CO-1020) and the National Institute of General Medical Sciences (Y1-GM-1104). Use of the Advanced Photon Source was supported by the U.S. Department of Energy, Basic Energy Sciences, Office of Science, under contract no. DE-AC02-06CH11357. Coordinates and data have been deposited in the Protein Data Bank with accession code 4ZUX. Plasmid pST55-16xNCP601 expressing the Widom 601 DNA fragment was obtained from S. Tan under a material transfer agreement with Pennsylvania State University. Publisher Copyright: {\textcopyright} 2016, American Association for the Advancement of Science. All rights reserved.",
year = "2016",
month = feb,
day = "12",
doi = "10.1126/science.aac5681",
language = "English",
volume = "351",
pages = "725--728",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6274",
}