Structural Similarity Enhances Interaction Propensity of Proteins

D. B. Lukatsky, B. E. Shakhnovich, J. Mintseris, E. I. Shakhnovich

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

We study statistical properties of interacting protein-like surfaces and predict two strong, related effects: (i) statistically enhanced self-attraction of proteins; (ii) statistically enhanced attraction of proteins with similar structures. The effects originate in the fact that the probability to find a pattern self-match between two identical, even randomly organized interacting protein surfaces is always higher compared with the probability for a pattern match between two different, promiscuous protein surfaces. This theoretical finding explains statistical prevalence of homodimers in protein-protein interaction networks reported earlier. Further, our findings are confirmed by the analysis of curated database of protein complexes that showed highly statistically significant overrepresentation of dimers formed by structurally similar proteins with highly divergent sequences ("superfamily heterodimers"). We suggest that promiscuous homodimeric interactions pose strong competitive interactions for heterodimers evolved from homodimers. Such evolutionary bottleneck is overcome using the negative design evolutionary pressure applied against promiscuous homodimer formation. This is achieved through the formation of highly specific contacts formed by charged residues as demonstrated both in model and real superfamily heterodimers.

Original languageEnglish
Pages (from-to)1596-1606
Number of pages11
JournalJournal of Molecular Biology
Volume365
Issue number5
DOIs
StatePublished - 2 Feb 2007
Externally publishedYes

Keywords

  • homodimers and heterodimers
  • positive and negative design
  • principles of biomolecular recognition
  • protein networks
  • protein-protein interactions

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Structural Similarity Enhances Interaction Propensity of Proteins'. Together they form a unique fingerprint.

Cite this