Structure and gating of the nuclear pore complex

Matthias Eibauer, Mauro Pellanda, Yagmur Turgay, Anna Dubrovsky, Annik Wild, Ohad Medalia

Research output: Contribution to journalArticlepeer-review

122 Scopus citations

Abstract

Nuclear pore complexes (NPCs) perforate the nuclear envelope and allow the exchange of macromolecules between the nucleus and the cytoplasm. To acquire a deeper understanding of this transport mechanism, we analyse the structure of the NPC scaffold and permeability barrier, by reconstructing the Xenopus laevis oocyte NPC from native nuclear envelopes up to 20â €‰Å resolution by cryo-electron tomography in conjunction with subtomogram averaging. In addition to resolving individual protein domains of the NPC constituents, we propose a model for the architecture of the molecular gate at its central channel. Furthermore, we compare and contrast this native NPC structure to one that exhibits reduced transport activity and unveil the spatial properties of the NPC gate.

Original languageEnglish
Article number7532
JournalNature Communications
Volume6
DOIs
StatePublished - 26 Jun 2015

ASJC Scopus subject areas

  • Chemistry (all)
  • Biochemistry, Genetics and Molecular Biology (all)
  • Physics and Astronomy (all)

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