TY - JOUR
T1 - Structure of DNA coils in dilute and semidilute solutions
AU - Nepal, Manish
AU - Yaniv, Alon
AU - Shafran, Eyal
AU - Krichevsky, Oleg
PY - 2013/1/29
Y1 - 2013/1/29
N2 - We apply scanning fluorescence correlation spectroscopy to study the structure of individual DNA coils in dilute and semidilute solutions. In dilute solutions, over two decades in length, from 0.6 to 46 μm, DNA behave as ideal chains, in agreement with theoretical predictions and in disagreement with prior experiments. In semidilute solutions, up to very high densities, the structures of individual DNA coils are independent of concentration, unlike flexible coils that shrink with increasing density. Our experimental findings are consistent with the marginal solution theory of semiflexible polymers.
AB - We apply scanning fluorescence correlation spectroscopy to study the structure of individual DNA coils in dilute and semidilute solutions. In dilute solutions, over two decades in length, from 0.6 to 46 μm, DNA behave as ideal chains, in agreement with theoretical predictions and in disagreement with prior experiments. In semidilute solutions, up to very high densities, the structures of individual DNA coils are independent of concentration, unlike flexible coils that shrink with increasing density. Our experimental findings are consistent with the marginal solution theory of semiflexible polymers.
UR - http://www.scopus.com/inward/record.url?scp=84873817479&partnerID=8YFLogxK
U2 - 10.1103/PhysRevLett.110.058102
DO - 10.1103/PhysRevLett.110.058102
M3 - Article
C2 - 23414049
AN - SCOPUS:84873817479
VL - 110
JO - Physical Review Letters
JF - Physical Review Letters
SN - 0031-9007
IS - 5
M1 - 058102
ER -