Structure of functionally activated small ribosomal subunit at 3.3 Å resolution

Frank Schluenzen, Ante Tocilj, Raz Zarivach, Joerg Harms, Marco Gluehmann, Daniela Janell, Anat Bashan, Heike Bartels, Ilana Agmon, François Franceschi, Ada Yonath

Research output: Contribution to journalArticlepeer-review

883 Scopus citations

Abstract

The small ribosomal subunit performs the decoding of genetic information during translation. The structure of that from Thermus thermophilus shows that the decoding center, which positions mRNA and three tRNAs, is constructed entirely of RNA. The entrance to the mRNA channel will encircle the message when a latch-like contact closes and contributes to processivity and fidelity. Extended RNA helical elements that run longitudinally through the body transmit structural changes, correlating events at the particle's far end with the cycle of mRNA translocation at the decoding region. 96% of the nucleotides were traced and the main fold of all proteins was determined. The latter are either peripheral or appear to serve as linkers. Some may assist the directionality of translocation.

Original languageEnglish
Pages (from-to)615-623
Number of pages9
JournalCell
Volume102
Issue number5
DOIs
StatePublished - 1 Sep 2000
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

Fingerprint

Dive into the research topics of 'Structure of functionally activated small ribosomal subunit at 3.3 Å resolution'. Together they form a unique fingerprint.

Cite this