TY - JOUR
T1 - Structure of functionally activated small ribosomal subunit at 3.3 Å resolution
AU - Schluenzen, Frank
AU - Tocilj, Ante
AU - Zarivach, Raz
AU - Harms, Joerg
AU - Gluehmann, Marco
AU - Janell, Daniela
AU - Bashan, Anat
AU - Bartels, Heike
AU - Agmon, Ilana
AU - Franceschi, François
AU - Yonath, Ada
N1 - Funding Information:
Raz Zarivach contributed significantly to the tracing, and Joerg Harms to the assignments. We thank S. Harrison and W. Traub for critical review of the manuscript, M. Wilchek for indispensable advice, M. Pope for heavy-atom compounds, R. Brimacombe and H. Stark for fruitful discussions. We thank R. Albrecht, T. Auerbach, H. Avila, W.S. Bennett, H. Burmeister, D. Choli, C. Glotz, H.A.S. Hansen, R. Kafri, K. Knaack, M. Laschever, S. Meier, J. Muessig, M. Pioletti, M. Peretz, C. Radzwill, I. Sagi, B. Schmidt, A. Vieweger, and S. Weinstein for contributing to different stages of these studies. These studies could not be performed without the cooperation of the staff of the synchrotron radiation facilities at EMBL & MPG beam lines at DESY; ID2 and ID14/2 and 4 at EMBL and ESRF; ID19/APS/ANL and F1/CHESS. Support was provided by the Max-Planck Society, the US National Institutes of Health (GM34360), the German Ministry for Science and Technology (Bundesministerium für Bildung, Wissenschaft, Forschung und Technologie Grant 05-641EA), and the Kimmelman Center for Macromolecular Assembly at the Weizmann Institute. A. Y. holds the Martin S. Kimmel Professorial Chair.
PY - 2000/9/1
Y1 - 2000/9/1
N2 - The small ribosomal subunit performs the decoding of genetic information during translation. The structure of that from Thermus thermophilus shows that the decoding center, which positions mRNA and three tRNAs, is constructed entirely of RNA. The entrance to the mRNA channel will encircle the message when a latch-like contact closes and contributes to processivity and fidelity. Extended RNA helical elements that run longitudinally through the body transmit structural changes, correlating events at the particle's far end with the cycle of mRNA translocation at the decoding region. 96% of the nucleotides were traced and the main fold of all proteins was determined. The latter are either peripheral or appear to serve as linkers. Some may assist the directionality of translocation.
AB - The small ribosomal subunit performs the decoding of genetic information during translation. The structure of that from Thermus thermophilus shows that the decoding center, which positions mRNA and three tRNAs, is constructed entirely of RNA. The entrance to the mRNA channel will encircle the message when a latch-like contact closes and contributes to processivity and fidelity. Extended RNA helical elements that run longitudinally through the body transmit structural changes, correlating events at the particle's far end with the cycle of mRNA translocation at the decoding region. 96% of the nucleotides were traced and the main fold of all proteins was determined. The latter are either peripheral or appear to serve as linkers. Some may assist the directionality of translocation.
UR - http://www.scopus.com/inward/record.url?scp=0034268836&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)00084-2
DO - 10.1016/S0092-8674(00)00084-2
M3 - Article
AN - SCOPUS:0034268836
SN - 0092-8674
VL - 102
SP - 615
EP - 623
JO - Cell
JF - Cell
IS - 5
ER -