Studies of coat protein-mediated resistance to tobacco mosaic virus (TMV). II. Challenge by a mutant with altered virion surface does not overcome resistance conferred by TMV coat protein

W. G. Clark, J. Fitchen, A. Nejidat, C. M. Deom, R. N. Beachy

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14 Scopus citations

Abstract

Transgenic tobacco plants expressing the coat protein (CP) gene of the U1 strain of tobacco mosaic virus (TMV) exhibit CP-mediated resistance (CP-MR) against some, but not all, tobamoviruses. To investigate the role of the amino acid sequences on the surface of the challenge virus in CP-MR, mutant strains of U1 TMV were constructed to contain the amino or carboxy termini of the CP of Sunn hemp mosaic tobamovirus (SHMV). The modified virus was unable to overcome CP-MR in transgenic plants that contained the TMV CP. In contrast, TMV in which the CP was replaced by the SHMV CP overcame CP-MR to the same extent as did SHMV. We conclude that CP-MR conferred by TMV CP involves interactions between amino acid sequences of the challenge viruses and the transgene protein other than those on the surface of the challenge virus.

Original languageEnglish
Pages (from-to)2613-2617
Number of pages5
JournalJournal of General Virology
Volume76
Issue number10
DOIs
StatePublished - 1 Jan 1995
Externally publishedYes

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