Studies of new two-photon fluorescent probes suitable for multi-photon microscopy in biological settings

Raz Gvishi; Garry Berkovic; Zvi Kotler; Pnina Krief; Lev Shapiro; Jacob T. Klug; Jacqueline Skorka; Vladimir Khodorkovsky

doi:10.1117/12.506766

Studies of new two-photon fluorescent probes suitable for multi-photon microscopy in biological settings

Raz Gvishi, Garry Berkovic, Zvi Kotler, Pnina Krief, Lev Shapiro, Jacob T. Klug, Jacqueline Skorka, Vladimir Khodorkovsky

Department of Chemistry

Research output: Contribution to journal › Conference article › peer-review

5 Scopus citations

Abstract

Multi-Photon Laser Scanning Microscopy (MPLSM) requires efficient two-photon absorbing fluorescent (TPF) probes. In particular, probes exhibiting bio-functionality are very attractive for MPLSM studies of biological samples. We have synthesized and studied a new class of TPF probes capable of caging metal ions, such as Ca⁺² and Na⁺, which play an important role in neuronal mechanisms. The TPF probes are based on a tetraketo derivative with a symmetric Donor-Acceptor-Donor (D-A-D) structure. The donor is an azacrown moiety, which also serves as a metal ion-caging unit. We studied the linear and the non-linear spectroscopic properties of these TPF probes as a function of conjugation length and the size of the crown ring. We find that this new class of TPF probes possesses very large two-photon excitation cross-section coefficients (∼1000GM) at near IR wavelengths as well as affinity to metal ions. In the presence of changing sodium ion concentration the dye spectra reveals four distinguishable forms and the TPF efficiency changes strongly. We therefore conclude that the dye can perform as a sensitive metal ion TPF probe.

Original language	English
Pages (from-to)	82-90
Number of pages	9
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5211
DOIs	https://doi.org/10.1117/12.506766
State	Published - 1 Dec 2003
Event	Nonlinear Optical Transmission and Multiphoton Processes in Organics - San Diego, CA, United States Duration: 3 Aug 2003 → 4 Aug 2003

Keywords

Conjugated molecules
Crown-ether
D-A-D
Fluorescent probes
MPLSM
Two-photon Absorption

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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title = "Studies of new two-photon fluorescent probes suitable for multi-photon microscopy in biological settings",

abstract = "Multi-Photon Laser Scanning Microscopy (MPLSM) requires efficient two-photon absorbing fluorescent (TPF) probes. In particular, probes exhibiting bio-functionality are very attractive for MPLSM studies of biological samples. We have synthesized and studied a new class of TPF probes capable of caging metal ions, such as Ca+2 and Na+, which play an important role in neuronal mechanisms. The TPF probes are based on a tetraketo derivative with a symmetric Donor-Acceptor-Donor (D-A-D) structure. The donor is an azacrown moiety, which also serves as a metal ion-caging unit. We studied the linear and the non-linear spectroscopic properties of these TPF probes as a function of conjugation length and the size of the crown ring. We find that this new class of TPF probes possesses very large two-photon excitation cross-section coefficients (∼1000GM) at near IR wavelengths as well as affinity to metal ions. In the presence of changing sodium ion concentration the dye spectra reveals four distinguishable forms and the TPF efficiency changes strongly. We therefore conclude that the dye can perform as a sensitive metal ion TPF probe.",

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author = "Raz Gvishi and Garry Berkovic and Zvi Kotler and Pnina Krief and Lev Shapiro and Klug, {Jacob T.} and Jacqueline Skorka and Vladimir Khodorkovsky",

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AU - Gvishi, Raz

AU - Berkovic, Garry

AU - Kotler, Zvi

AU - Krief, Pnina

AU - Shapiro, Lev

AU - Klug, Jacob T.

AU - Skorka, Jacqueline

AU - Khodorkovsky, Vladimir

PY - 2003/12/1

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N2 - Multi-Photon Laser Scanning Microscopy (MPLSM) requires efficient two-photon absorbing fluorescent (TPF) probes. In particular, probes exhibiting bio-functionality are very attractive for MPLSM studies of biological samples. We have synthesized and studied a new class of TPF probes capable of caging metal ions, such as Ca+2 and Na+, which play an important role in neuronal mechanisms. The TPF probes are based on a tetraketo derivative with a symmetric Donor-Acceptor-Donor (D-A-D) structure. The donor is an azacrown moiety, which also serves as a metal ion-caging unit. We studied the linear and the non-linear spectroscopic properties of these TPF probes as a function of conjugation length and the size of the crown ring. We find that this new class of TPF probes possesses very large two-photon excitation cross-section coefficients (∼1000GM) at near IR wavelengths as well as affinity to metal ions. In the presence of changing sodium ion concentration the dye spectra reveals four distinguishable forms and the TPF efficiency changes strongly. We therefore conclude that the dye can perform as a sensitive metal ion TPF probe.

AB - Multi-Photon Laser Scanning Microscopy (MPLSM) requires efficient two-photon absorbing fluorescent (TPF) probes. In particular, probes exhibiting bio-functionality are very attractive for MPLSM studies of biological samples. We have synthesized and studied a new class of TPF probes capable of caging metal ions, such as Ca+2 and Na+, which play an important role in neuronal mechanisms. The TPF probes are based on a tetraketo derivative with a symmetric Donor-Acceptor-Donor (D-A-D) structure. The donor is an azacrown moiety, which also serves as a metal ion-caging unit. We studied the linear and the non-linear spectroscopic properties of these TPF probes as a function of conjugation length and the size of the crown ring. We find that this new class of TPF probes possesses very large two-photon excitation cross-section coefficients (∼1000GM) at near IR wavelengths as well as affinity to metal ions. In the presence of changing sodium ion concentration the dye spectra reveals four distinguishable forms and the TPF efficiency changes strongly. We therefore conclude that the dye can perform as a sensitive metal ion TPF probe.

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ER -

Studies of new two-photon fluorescent probes suitable for multi-photon microscopy in biological settings

Abstract

Keywords

ASJC Scopus subject areas

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