Studies of new two-photon fluorescent probes suitable for multi-photon microscopy in biological settings

Raz Gvishi, Garry Berkovic, Zvi Kotler, Pnina Krief, Lev Shapiro, Jacob T. Klug, Jacqueline Skorka, Vladimir Khodorkovsky

Research output: Contribution to journalConference articlepeer-review

5 Scopus citations


Multi-Photon Laser Scanning Microscopy (MPLSM) requires efficient two-photon absorbing fluorescent (TPF) probes. In particular, probes exhibiting bio-functionality are very attractive for MPLSM studies of biological samples. We have synthesized and studied a new class of TPF probes capable of caging metal ions, such as Ca+2 and Na+, which play an important role in neuronal mechanisms. The TPF probes are based on a tetraketo derivative with a symmetric Donor-Acceptor-Donor (D-A-D) structure. The donor is an azacrown moiety, which also serves as a metal ion-caging unit. We studied the linear and the non-linear spectroscopic properties of these TPF probes as a function of conjugation length and the size of the crown ring. We find that this new class of TPF probes possesses very large two-photon excitation cross-section coefficients (∼1000GM) at near IR wavelengths as well as affinity to metal ions. In the presence of changing sodium ion concentration the dye spectra reveals four distinguishable forms and the TPF efficiency changes strongly. We therefore conclude that the dye can perform as a sensitive metal ion TPF probe.

Original languageEnglish
Pages (from-to)82-90
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 1 Dec 2003
EventNonlinear Optical Transmission and Multiphoton Processes in Organics - San Diego, CA, United States
Duration: 3 Aug 20034 Aug 2003


  • Conjugated molecules
  • Crown-ether
  • D-A-D
  • Fluorescent probes
  • Two-photon Absorption

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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