Studies on Acute Human Infections Using FTIR Microspectroscopy and Cluster Analysis

Jacov Mordehai; Jagannathan Ramesh; Mahmoud Huleihel; Zahavi Cohen; Oleg Kleiner; Marina Talyshinsky; Vitaly Erukhimovitch; Aviv Cahana; Ahmad Salman; Ranjit Kumar Sahu; Hugo Guterman; Shaul Mordechai

doi:10.1002/bip.10554

Studies on Acute Human Infections Using FTIR Microspectroscopy and Cluster Analysis

Jacov Mordehai, Jagannathan Ramesh, Mahmoud Huleihel, Zahavi Cohen, Oleg Kleiner, Marina Talyshinsky, Vitaly Erukhimovitch, Aviv Cahana, Ahmad Salman, Ranjit Kumar Sahu, Hugo Guterman, Shaul Mordechai

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

A novel methodology for the diagnosis of acute infections using FTIR microspectroscopy (FTIR-MSP) data on blood components and cluster analysis is presented. Blood samples were collected from 11 patients suffering from various infections and 16 age-matched healthy human controls. Blood components such as white blood cells, red blood cells, and plasma were isolated using standard procedures and FTIR-MSP of these components was utilized. A cluster analysis of the FTIR spectra was performed. The spectra obtained from the three blood components of patients were different from those of controls. The FTIR spectra of white blood cells from patients suffering infections were significantly different from the controls. Cluster analyses of averaged FTIR-MSP spectra of white blood cells provided 100% classification between patients and healthy controls.

Original language	English
Pages (from-to)	494-502
Number of pages	9
Journal	Biopolymers
Volume	73
Issue number	4
DOIs	https://doi.org/10.1002/bip.10554
State	Published - 1 Mar 2004

Keywords

Blood
Cluster analysis
Diagnosis
FTIR microspectroscopy
Infection

ASJC Scopus subject areas

Biophysics
Biochemistry
Biomaterials
Organic Chemistry

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10.1002/bip.10554

Cite this

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title = "Studies on Acute Human Infections Using FTIR Microspectroscopy and Cluster Analysis",

abstract = "A novel methodology for the diagnosis of acute infections using FTIR microspectroscopy (FTIR-MSP) data on blood components and cluster analysis is presented. Blood samples were collected from 11 patients suffering from various infections and 16 age-matched healthy human controls. Blood components such as white blood cells, red blood cells, and plasma were isolated using standard procedures and FTIR-MSP of these components was utilized. A cluster analysis of the FTIR spectra was performed. The spectra obtained from the three blood components of patients were different from those of controls. The FTIR spectra of white blood cells from patients suffering infections were significantly different from the controls. Cluster analyses of averaged FTIR-MSP spectra of white blood cells provided 100% classification between patients and healthy controls.",

keywords = "Blood, Cluster analysis, Diagnosis, FTIR microspectroscopy, Infection",

author = "Jacov Mordehai and Jagannathan Ramesh and Mahmoud Huleihel and Zahavi Cohen and Oleg Kleiner and Marina Talyshinsky and Vitaly Erukhimovitch and Aviv Cahana and Ahmad Salman and Sahu, {Ranjit Kumar} and Hugo Guterman and Shaul Mordechai",

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doi = "10.1002/bip.10554",

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T1 - Studies on Acute Human Infections Using FTIR Microspectroscopy and Cluster Analysis

AU - Mordehai, Jacov

AU - Ramesh, Jagannathan

AU - Huleihel, Mahmoud

AU - Cohen, Zahavi

AU - Kleiner, Oleg

AU - Talyshinsky, Marina

AU - Erukhimovitch, Vitaly

AU - Cahana, Aviv

AU - Salman, Ahmad

AU - Sahu, Ranjit Kumar

AU - Guterman, Hugo

AU - Mordechai, Shaul

PY - 2004/3/1

Y1 - 2004/3/1

N2 - A novel methodology for the diagnosis of acute infections using FTIR microspectroscopy (FTIR-MSP) data on blood components and cluster analysis is presented. Blood samples were collected from 11 patients suffering from various infections and 16 age-matched healthy human controls. Blood components such as white blood cells, red blood cells, and plasma were isolated using standard procedures and FTIR-MSP of these components was utilized. A cluster analysis of the FTIR spectra was performed. The spectra obtained from the three blood components of patients were different from those of controls. The FTIR spectra of white blood cells from patients suffering infections were significantly different from the controls. Cluster analyses of averaged FTIR-MSP spectra of white blood cells provided 100% classification between patients and healthy controls.

AB - A novel methodology for the diagnosis of acute infections using FTIR microspectroscopy (FTIR-MSP) data on blood components and cluster analysis is presented. Blood samples were collected from 11 patients suffering from various infections and 16 age-matched healthy human controls. Blood components such as white blood cells, red blood cells, and plasma were isolated using standard procedures and FTIR-MSP of these components was utilized. A cluster analysis of the FTIR spectra was performed. The spectra obtained from the three blood components of patients were different from those of controls. The FTIR spectra of white blood cells from patients suffering infections were significantly different from the controls. Cluster analyses of averaged FTIR-MSP spectra of white blood cells provided 100% classification between patients and healthy controls.

KW - Blood

KW - Cluster analysis

KW - Diagnosis

KW - FTIR microspectroscopy

KW - Infection

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U2 - 10.1002/bip.10554

DO - 10.1002/bip.10554

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SN - 0006-3525

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SP - 494

EP - 502

JO - Biopolymers

JF - Biopolymers

IS - 4

ER -

Studies on Acute Human Infections Using FTIR Microspectroscopy and Cluster Analysis

Abstract

Keywords

ASJC Scopus subject areas

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