TY - JOUR
T1 - Studies on production and biological potential of prodigiosin by Serratia marcescens
AU - Suryawanshi, Rahul K.
AU - Patil, Chandrashekhar D.
AU - Borase, Hemant P.
AU - Salunke, Bipinchandra K.
AU - Patil, Satish V.
N1 - Funding Information:
Acknowledgments The author RKS is thankful to UGC BSR RSSMS scheme (Ref.: F.7-137/2007), New Delhi, India for the financial assistance. We are also thankful to North Maharashtra culture collection (NMCC) for providing the microbial cultures.
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Efficacy of Serratia marcescens for pigment production and biological activity was investigated. Natural substrates like sweet potato, mahua flower extract (Madhuca latifolia L.), and sesam at different concentrations were taken. As a carbon source microorganism favored potato powder was followed by sesam and mannitol, and as nitrogen source casein hydrolysate was followed by yeast and malt extract. The effect of inorganic salts on pigment production was also studied. At final optimized composition of suitable carbon, nitrogen source, and trace materials and at suitable physiological conditions, prodigiosin production was 4.8 g L-1. The isolated pigment showed antimicrobial activity against different pathogenic bacteria and fungi. Extracted pigment was characterized by spectroscopy, Fourier transform infrared (FTIR), and thin layer chromatography (TLC) which confirm production of biological compound prodigiosin. This study suggests that use of sweet potato powder and casein can be a potential alternative bioresource for commercial production of pigment prodigiosin.
AB - Efficacy of Serratia marcescens for pigment production and biological activity was investigated. Natural substrates like sweet potato, mahua flower extract (Madhuca latifolia L.), and sesam at different concentrations were taken. As a carbon source microorganism favored potato powder was followed by sesam and mannitol, and as nitrogen source casein hydrolysate was followed by yeast and malt extract. The effect of inorganic salts on pigment production was also studied. At final optimized composition of suitable carbon, nitrogen source, and trace materials and at suitable physiological conditions, prodigiosin production was 4.8 g L-1. The isolated pigment showed antimicrobial activity against different pathogenic bacteria and fungi. Extracted pigment was characterized by spectroscopy, Fourier transform infrared (FTIR), and thin layer chromatography (TLC) which confirm production of biological compound prodigiosin. This study suggests that use of sweet potato powder and casein can be a potential alternative bioresource for commercial production of pigment prodigiosin.
KW - Optimization
KW - Prodigiosin
KW - Production
KW - Serratia
UR - http://www.scopus.com/inward/record.url?scp=84904857609&partnerID=8YFLogxK
U2 - 10.1007/s12010-014-0921-3
DO - 10.1007/s12010-014-0921-3
M3 - Article
AN - SCOPUS:84904857609
SN - 0273-2289
VL - 173
SP - 1209
EP - 1221
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 5
ER -