TY - JOUR
T1 - Studies on the Intracellular Location of Enzymes of the Photosynthetic Carbon‐Reduction Cycle
AU - KAGAN‐ZUR, Varda
AU - Lips, Herman
PY - 1975/1/1
Y1 - 1975/1/1
N2 - Homogenates of dark‐pretreated leaves yield two particulate fractions in density gradient centrifugation: one contains chlorophyll (chloroplasts) while a second fraction contains ribulose‐1,5‐bisphosphate carboxylase, NADP‐dependent glyceraldehyde‐3‐phosphate dehydrogenase and catalase. Addition of a microbody‐rich pellet to chloroplasts isolated from dark‐pretreated plants largely enhances both oxygen evolution and CO2‐fixation into organic compounds. The pathway of CO2 reduction may be part of a membrane system which, under suitable conditions, may separate from the chloroplast as a distinct cytoplasmic entity, having physical properties similar to those of microbodies.
AB - Homogenates of dark‐pretreated leaves yield two particulate fractions in density gradient centrifugation: one contains chlorophyll (chloroplasts) while a second fraction contains ribulose‐1,5‐bisphosphate carboxylase, NADP‐dependent glyceraldehyde‐3‐phosphate dehydrogenase and catalase. Addition of a microbody‐rich pellet to chloroplasts isolated from dark‐pretreated plants largely enhances both oxygen evolution and CO2‐fixation into organic compounds. The pathway of CO2 reduction may be part of a membrane system which, under suitable conditions, may separate from the chloroplast as a distinct cytoplasmic entity, having physical properties similar to those of microbodies.
UR - http://www.scopus.com/inward/record.url?scp=0016830763&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1975.tb02419.x
DO - 10.1111/j.1432-1033.1975.tb02419.x
M3 - Article
AN - SCOPUS:0016830763
SN - 0014-2956
VL - 59
SP - 17
EP - 23
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 1
ER -