Studies on the Intracellular Location of Enzymes of the Photosynthetic Carbon‐Reduction Cycle

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    6 Scopus citations

    Abstract

    Homogenates of dark‐pretreated leaves yield two particulate fractions in density gradient centrifugation: one contains chlorophyll (chloroplasts) while a second fraction contains ribulose‐1,5‐bisphosphate carboxylase, NADP‐dependent glyceraldehyde‐3‐phosphate dehydrogenase and catalase. Addition of a microbody‐rich pellet to chloroplasts isolated from dark‐pretreated plants largely enhances both oxygen evolution and CO2‐fixation into organic compounds. The pathway of CO2 reduction may be part of a membrane system which, under suitable conditions, may separate from the chloroplast as a distinct cytoplasmic entity, having physical properties similar to those of microbodies.

    Original languageEnglish
    Pages (from-to)17-23
    Number of pages7
    JournalEuropean Journal of Biochemistry
    Volume59
    Issue number1
    DOIs
    StatePublished - 1 Jan 1975

    ASJC Scopus subject areas

    • Biochemistry

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