Homogenates of dark‐pretreated leaves yield two particulate fractions in density gradient centrifugation: one contains chlorophyll (chloroplasts) while a second fraction contains ribulose‐1,5‐bisphosphate carboxylase, NADP‐dependent glyceraldehyde‐3‐phosphate dehydrogenase and catalase. Addition of a microbody‐rich pellet to chloroplasts isolated from dark‐pretreated plants largely enhances both oxygen evolution and CO2‐fixation into organic compounds. The pathway of CO2 reduction may be part of a membrane system which, under suitable conditions, may separate from the chloroplast as a distinct cytoplasmic entity, having physical properties similar to those of microbodies.
|Number of pages||7|
|Journal||European Journal of Biochemistry|
|State||Published - 1 Jan 1975|
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