TY - JOUR
T1 - Study of silver nanoparticle-hemoglobin interaction and composite formation
AU - Mahato, Mrityunjoy
AU - Pal, Prabir
AU - Tah, Bidisha
AU - Ghosh, Manash
AU - Talapatra, G. B.
N1 - Funding Information:
We thank DST, Government of India (Project No. SR/S2/CMP-0051/2006) for partial financial support. Mrityunjoy Mahato thanks CSIR, Government of India for providing the CSIR-NET fellowship. Subrata Das is acknowledged for his technical assistant in fitting the lifetime data.
PY - 2011/11/1
Y1 - 2011/11/1
N2 - Nanoscience is now an expanding field of research and finds potential application in biomedical area, but it is limited due to lack of comprehensive knowledge of the interactions operating in nano-bio system. Here, we report the studies on the interaction and formation of nano-bio complex between silver nanoparticle (AgNP) and human blood protein hemoglobin (Hb). We have employed several spectroscopic (absorption, emission, Raman, FTIR, CD, etc.) and electron diffraction techniques (FE-SEM and HR-TEM) to characterize the Hb-AgNP complex system. Our results show the Hb-AgNP interaction is concentration and time dependent. The AgNP particle can attach/come closer to heme, tryptophan, and amide as well aromatic amine residues. As a result, the Hb undergoes conformational change and becomes unfolded through the increment of β-sheet structure. The AgNP-Hb can form charge-transfers (CT) complex where the Hb-heme along with the AgNP involved in the electron transfer mechanism and form Hb-AgNP assembled structure. The electron transfer mechanism has been found to be dependent on the size of silver particle. The overall study is important in understanding the nano-bio system and in predicting the avenues to design and synthesis of novel nano-biocomposite materials in material science and biomedical area.
AB - Nanoscience is now an expanding field of research and finds potential application in biomedical area, but it is limited due to lack of comprehensive knowledge of the interactions operating in nano-bio system. Here, we report the studies on the interaction and formation of nano-bio complex between silver nanoparticle (AgNP) and human blood protein hemoglobin (Hb). We have employed several spectroscopic (absorption, emission, Raman, FTIR, CD, etc.) and electron diffraction techniques (FE-SEM and HR-TEM) to characterize the Hb-AgNP complex system. Our results show the Hb-AgNP interaction is concentration and time dependent. The AgNP particle can attach/come closer to heme, tryptophan, and amide as well aromatic amine residues. As a result, the Hb undergoes conformational change and becomes unfolded through the increment of β-sheet structure. The AgNP-Hb can form charge-transfers (CT) complex where the Hb-heme along with the AgNP involved in the electron transfer mechanism and form Hb-AgNP assembled structure. The electron transfer mechanism has been found to be dependent on the size of silver particle. The overall study is important in understanding the nano-bio system and in predicting the avenues to design and synthesis of novel nano-biocomposite materials in material science and biomedical area.
KW - AgNP
KW - Biomedical
KW - Biophysiochemical interaction
KW - Conformational change
KW - Hemoglobin
KW - Nano-bio
KW - Nano-biocomposite material
UR - http://www.scopus.com/inward/record.url?scp=80052087725&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2011.06.024
DO - 10.1016/j.colsurfb.2011.06.024
M3 - Article
C2 - 21788120
AN - SCOPUS:80052087725
SN - 0927-7765
VL - 88
SP - 141
EP - 149
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
IS - 1
ER -