Subcellular localization of VDAC in mitochondria and ER in the cerebellum

Varda Shoshan-Barmatz, Ran Zalk, Dan Gincel, Noga Vardi

Research output: Contribution to journalArticlepeer-review

92 Scopus citations


The voltage-dependent anion channel (VDAC) provides passage for adenine nucleotides, Ca2+ and other metabolites into and from mitochondria. Here, the intracellular localization and oligomeric organization of VDAC in brain mitochondria and ER are demonstrated. Immunohistochemical staining of VDAC in rat cerebellum showed high labeling of the Purkinje neurons. Immunogold labeling and EM analysis of the cerebellar molecular layer showed specific VDAC immunostaining of the mitochondrial outer membrane, highly enhanced in contact sites between mitochondria or between mitochondria and associated ER. Purified ER membranes contain VDAC, but not other mitochondrial proteins. Chemical cross-linking of isolated mitochondria, ER or purified VDAC demonstrated the existence of VDAC in oligomeric form. Based on the enrichment of VDAC in the junctional face of closely associated mitochondrial and ER membranes and the existence of VDAC oligomers, we propose an involvement of VDAC in specialized intermembrane communication between mitochondria or between ER and mitochondria, serving to complement the tight structural and functional coupling observed between these organelles.

Original languageEnglish
Pages (from-to)105-114
Number of pages10
JournalBiochimica et Biophysica Acta - Bioenergetics
Issue number2-3
StatePublished - 9 Jul 2004


  • 3,3′-diamino-benzidine tetrahydrochloride
  • Cerebellum
  • DAB
  • DMS
  • DSP
  • EGS
  • EGTA
  • ER and mitochondria
  • VDAC
  • dimethylsuberimidate
  • dithiobis(succinimidylpropionate)
  • ethylene glycol bis (aminoethylether) tetraacetate

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology


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