Substitution by Inosine at the 3′-Ultimate and Penultimate Positions of 16S rRNA Gene Universal Primers

Eitan Ben-Dov, Nachshon Siboni, Orr H. Shapiro, Luba Arotsker, Ariel Kushmaro

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Universal 16S rRNA gene primers (8F and 518R) bearing inosine substitutions at either the 3′-ultimate or the 3′-ultimate and penultimate base positions were exploited for the first time to study the bacterial community associated with coral polymicrobial Black Band Disease (BBD). Inosine-modified universal primer pairs display some shifting in the composition of 16S rRNA gene libraries, as well as expanding the observed diversity of a BBD bacterial community at the family/class level. Possible explanations for the observed shifts are discussed. These results thus point to the need for adopting multiple approaches in designing 16S rRNA universal primers for PCR amplification and subsequent construction of 16S rRNA gene libraries or pyrosequencing in the exploration of complex microbial communities.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalMicrobial Ecology
Volume61
Issue number1
DOIs
StatePublished - 1 Jan 2011

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Soil Science

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