Substrate inhibition of the (Na+, K+)-ATPase in the presence of excess Mg2+

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1. 1. High concentrations of ATP inhibit completely the activity of (Na+, K+)-ATPase (ATP phosphohydrolase, EC prepared from sheep brain. 2. 2. The inhibition depends on the concentration of total ATP, i.e. complexed ATP + free ATP. 3. 3. The inhibition by high ATP concentrations persists in the absence of K+, and is then independent of the Na+ concentration between 2 and 140 mM Na+. 4. 4. Raising the K+ concentration at 20 mM Na+ increases the ATP concentration required for the maximal hydrolysis rate. 5. 5. The Hill number for the inhibition process is about three. 6. 6. The inhibition by ATP is temperature-dependent, in that as the temperature is increased. higher ATP concentrations are required for inhibition.

Original languageEnglish
Pages (from-to)434-445
Number of pages12
JournalBBA - Enzymology
Issue number2
StatePublished - 8 Dec 1977
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine


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