TY - JOUR
T1 - Sugar Synthesis from CO2 in Escherichia coli
AU - Antonovsky, Niv
AU - Gleizer, Shmuel
AU - Noor, Elad
AU - Zohar, Yehudit
AU - Herz, Elad
AU - Barenholz, Uri
AU - Zelcbuch, Lior
AU - Amram, Shira
AU - Wides, Aryeh
AU - Tepper, Naama
AU - Davidi, Dan
AU - Bar-On, Yinon
AU - Bareia, Tasneem
AU - Wernick, David G
AU - Shani, Ido
AU - Malitsky, Sergey
AU - Jona, Ghil
AU - Bar-Even, Arren
AU - Milo, Ron
N1 - Funding Information:
We thank Ichiro Matsumura for kindly supplying the prkA plasmid and Michal Shapira for kindly supplying the R. rubrum culture. We also thank Amir Aharoni, Asaph Aharoni, Uri Alon, Gil Amitai, Abdussalam Azem, Naama Barkai, Sima Benjamin, Sonjia Billerbeck, Dvora Biran, Elad Chomski, Ahuva Cooperstein, Daniel Dar, Michal Dayagi, Shani Doron, Noa Dvir, Avigdor Eldar, David Fell, Avi Flamholz, Shay Fleishon, Idan Frumkin, Shlomit Gilad, Pierre Goloubinoff, Michael Gurevitz, Jacob Hanna, Tami Hayon, Lee-Or Herzog, Keren Kahil, Miriam Kaltenbach, Mechael Kanovsky, Aaron Kaplan, Leeat Keren, Roy Kishony, Hagar Kroytoro, Rob Last, Paola Laurino, Tali Lavy, Ronen Levi, Ayelet Levin, Yishai Levin, Avi Levy, Hannes Link, Adar Lopez, Yehuda Marcus, Barak Marcus, Adi Millman, Uri Moran, Oliver Mueller-Cajar, Yotam Nadav, Amnon Naziri, Ravit Netzer, Gal Ofir, Nigel Orme, Rob Phillips, Uri Pick, Noa Rippel, Orel Rivni, Liat Rockah, Ilana Rogachev, Eliora Ron, Dave Savage, Alon Savidor, Efrat Schwartz, Lior Shachar, Tali Shalit, Tomer Shlomi, Rotem Sorek, Zvika Tamari, Dan Tawfik, Noam Vardi, Adi Volpert, Alon Wellner, Sagit Yahav, Dan Yakir, Oren Yishai, and Yonatan Zegman for support and feedback on this research. This work was funded by the European Research Council (projects SYMPAC 260392 and NOVCARBFIX 646827), Dana and Yossie Hollander, the Helmsley Charitable Foundation, the Larson Charitable Foundation, the Estate of David Arthur Barton, the Anthony Stalbow Charitable Trust, and Stella Gelerman, Canada. R.M. is the Charles and Louise Gartner professional chair and an EMBO young investigator program member. D.G.W. is supported by the United States-Israel Education Foundation.
Publisher Copyright:
© 2016 The Author(s)
PY - 2016/6/30
Y1 - 2016/6/30
N2 - Can a heterotrophic organism be evolved to synthesize biomass from CO2 directly? So far, non-native carbon fixation in which biomass precursors are synthesized solely from CO2 has remained an elusive grand challenge. Here, we demonstrate how a combination of rational metabolic rewiring, recombinant expression, and laboratory evolution has led to the biosynthesis of sugars and other major biomass constituents by a fully functional Calvin-Benson-Bassham (CBB) cycle in E. coli. In the evolved bacteria, carbon fixation is performed via a non-native CBB cycle, while reducing power and energy are obtained by oxidizing a supplied organic compound (e.g., pyruvate). Genome sequencing reveals that mutations in flux branchpoints, connecting the non-native CBB cycle to biosynthetic pathways, are essential for this phenotype. The successful evolution of a non-native carbon fixation pathway, though not yet resulting in net carbon gain, strikingly demonstrates the capacity for rapid trophic-mode evolution of metabolism applicable to biotechnology. PaperClip
AB - Can a heterotrophic organism be evolved to synthesize biomass from CO2 directly? So far, non-native carbon fixation in which biomass precursors are synthesized solely from CO2 has remained an elusive grand challenge. Here, we demonstrate how a combination of rational metabolic rewiring, recombinant expression, and laboratory evolution has led to the biosynthesis of sugars and other major biomass constituents by a fully functional Calvin-Benson-Bassham (CBB) cycle in E. coli. In the evolved bacteria, carbon fixation is performed via a non-native CBB cycle, while reducing power and energy are obtained by oxidizing a supplied organic compound (e.g., pyruvate). Genome sequencing reveals that mutations in flux branchpoints, connecting the non-native CBB cycle to biosynthetic pathways, are essential for this phenotype. The successful evolution of a non-native carbon fixation pathway, though not yet resulting in net carbon gain, strikingly demonstrates the capacity for rapid trophic-mode evolution of metabolism applicable to biotechnology. PaperClip
UR - http://www.scopus.com/inward/record.url?scp=84976892364&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2016.05.064
DO - 10.1016/j.cell.2016.05.064
M3 - Article
C2 - 27345370
AN - SCOPUS:84976892364
VL - 166
SP - 115
EP - 125
JO - Cell
JF - Cell
SN - 0092-8674
IS - 1
ER -