TY - JOUR
T1 - Suitability of Anabaena PCC7120 expressing mosquitocidal toxin genes from Bacillus thuringiensis subsp. israelensis for biotechnological application
AU - Lluisma, A. O.
AU - Karmacharya, N.
AU - Zarka, A.
AU - Ben-Dov, E.
AU - Zaritsky, A.
AU - Boussiba, S.
N1 - Funding Information:
Acknowledgements This work was partially supported by a grant from the United States–Israel Binational Science Foundation (No. 97–00081). The support of the Blaustein Center for Scientific Cooperation, Blaustein Institute for Desert Research (via a postdoctoral fellowship to A.O.L) is also acknowledged. Experiments conducted in connection with this report comply with the laws of the State of Israel.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - We present evidence that Anabaena PCC7120 (A.7120) strains expressing mosquitocidal toxin genes from Bacillus thuringiensis subsp. israelensis (Bti) have a strong potential for biotechnological application. Characterization of two 4-year-old recombinant A.7120 clones constructed previously in our laboratory [clone 7 and clone 11, each carrying three Bti genes (cry4Aa, cry11Aa, and p20)] revealed three facts. First, the Bti genes were stable in A.7120 even in the absence of antibiotic selection when the genes were integrated in the chromosome (in clone 11); and the genes were also stable as plasmid-borne constructs (in clone 7), provided the cultures were maintained under continued selection. Second, clone 7 (kept under selection) and clone 11 (either kept or not kept under selection) continued to be mosquitocidal through 4 years of culture. Third, growth of the recombinant clones was comparable to the wild type under optimal growth conditions, indicating that growth was not compromised by the expression of toxin genes. These results clear the way for the development of mass production techniques for A.7120 strains expressing Bti toxin genes.
AB - We present evidence that Anabaena PCC7120 (A.7120) strains expressing mosquitocidal toxin genes from Bacillus thuringiensis subsp. israelensis (Bti) have a strong potential for biotechnological application. Characterization of two 4-year-old recombinant A.7120 clones constructed previously in our laboratory [clone 7 and clone 11, each carrying three Bti genes (cry4Aa, cry11Aa, and p20)] revealed three facts. First, the Bti genes were stable in A.7120 even in the absence of antibiotic selection when the genes were integrated in the chromosome (in clone 11); and the genes were also stable as plasmid-borne constructs (in clone 7), provided the cultures were maintained under continued selection. Second, clone 7 (kept under selection) and clone 11 (either kept or not kept under selection) continued to be mosquitocidal through 4 years of culture. Third, growth of the recombinant clones was comparable to the wild type under optimal growth conditions, indicating that growth was not compromised by the expression of toxin genes. These results clear the way for the development of mass production techniques for A.7120 strains expressing Bti toxin genes.
UR - http://www.scopus.com/inward/record.url?scp=0034784558&partnerID=8YFLogxK
U2 - 10.1007/s002530100776
DO - 10.1007/s002530100776
M3 - Article
AN - SCOPUS:0034784558
SN - 0175-7598
VL - 57
SP - 161
EP - 166
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 1-2
ER -