TY - JOUR
T1 - Sulfite oxidase activity level determines the sulfite toxicity effect in leaves and fruits of tomato plants
AU - Sharma, Umanath
AU - Bekturova, Aizat
AU - Ventura, Yvonne
AU - Sagi, Moshe
N1 - Publisher Copyright:
© 2020 by the authors.
PY - 2020/5/1
Y1 - 2020/5/1
N2 - Increasing plant tolerance to sulfites/SO2 can lead to the development of tolerant crops to biotic and abiotic stresses. Plant sulfite oxidase (SO) is a molybdo-enzyme that oxidizes excess SO2/sulfite into non-toxic sulfate. The effect of toxic sulfite on leaves and fruits was studied in tomato plants with different SO expression: wild-type, SO overexpression (OE) and SO RNA interference (Ri). Sulfite-dipped ripe-fruits and sulfite treated leaf discs of Ri plants impaired in SO activity were more susceptible, whereas OE plants were more resistant, as revealed by remaining chlorophyll and tissue damage levels. Application of molybdenum further enhanced the tolerance of leaf discs to sulfite by enhancing SO activity in OE lines, but not in wild-type or Ri plants. Notably, incubation with tungsten, the molybdenum antagonist, overturned the effect of molybdenum spray in OE plants, revealed by remaining chlorophyll content and SO activity. The results indicate that SO in tomato leaves and ripe fruits determines the resistance to sulfite and the application of molybdenum enhances sulfite resistance in OE plants by increasing SO activity. Overall, the results suggest that SO overexpression can be employed, with or without molybdenum application, for developing fruit and vegetable crops tolerant to sulfite/SO2 containing pre- and postharvest treatments.
AB - Increasing plant tolerance to sulfites/SO2 can lead to the development of tolerant crops to biotic and abiotic stresses. Plant sulfite oxidase (SO) is a molybdo-enzyme that oxidizes excess SO2/sulfite into non-toxic sulfate. The effect of toxic sulfite on leaves and fruits was studied in tomato plants with different SO expression: wild-type, SO overexpression (OE) and SO RNA interference (Ri). Sulfite-dipped ripe-fruits and sulfite treated leaf discs of Ri plants impaired in SO activity were more susceptible, whereas OE plants were more resistant, as revealed by remaining chlorophyll and tissue damage levels. Application of molybdenum further enhanced the tolerance of leaf discs to sulfite by enhancing SO activity in OE lines, but not in wild-type or Ri plants. Notably, incubation with tungsten, the molybdenum antagonist, overturned the effect of molybdenum spray in OE plants, revealed by remaining chlorophyll content and SO activity. The results indicate that SO in tomato leaves and ripe fruits determines the resistance to sulfite and the application of molybdenum enhances sulfite resistance in OE plants by increasing SO activity. Overall, the results suggest that SO overexpression can be employed, with or without molybdenum application, for developing fruit and vegetable crops tolerant to sulfite/SO2 containing pre- and postharvest treatments.
KW - Molybdenum
KW - SO expression
KW - Sulfite oxidase
KW - Sulfite toxicity
KW - Tomato
KW - Tungsten
UR - http://www.scopus.com/inward/record.url?scp=85085467148&partnerID=8YFLogxK
U2 - 10.3390/agronomy10050694
DO - 10.3390/agronomy10050694
M3 - Article
AN - SCOPUS:85085467148
SN - 2073-4395
VL - 10
JO - Agronomy
JF - Agronomy
IS - 5
M1 - 694
ER -