15N NUCLEAR MAGNETIC RESONANCE STUDY OF AMMONIUM ION ASSIMILATION BY LEMNA GIBBA L.

EDNA BEN‐IZHAK MONSELISE, DANIEL KOST, DAN PORATH, MOSHE TAL

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13 Scopus citations

Abstract

15N Nuclear magnetic resonance (NMR) spectroscopy was used to follow nitrogen assimilation and amino acid production in Lemna gibba L. exposed to 0.4 mM 15NH4Cl solution for 24 h. NMR analysis of the 16N content of the treated plant tissues (aqueous extract) revealed 15N incorporation into glutamine (N‐amide plus amino‐N or amide‐N only) and glutamic acid and no detectable free ammonium ion. Methionine sulphoximine (MSO), an inhibitor of glutamine synthetase, at 1.0 mM inhibited completely the incorporation of 15N. In the presence of 0.5 mM azaserine (AZA), a glutamate synthase inhibitor, the incorporation of 15N was detected only in the amide group of glutamine. The results confirm the involvement of the glutamine synthetase/glutamate synthase (GS/ GOG AT) pathway in the assimilation of ammonium ions.

Original languageEnglish
Pages (from-to)341-345
Number of pages5
JournalNew Phytologist
Volume107
Issue number2
DOIs
StatePublished - 1 Jan 1987

Keywords

  • GS/GOGAT pathway
  • Lemna gibba
  • MSO and AZA
  • N‐NMR
  • ammonium ion assimilation

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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